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Applications of plant tissue and cell culture in the study of physiology of parasitism

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Proceedings / Indian Academy of Sciences

Abstract

The technique of growing plant tissues and cells on nutrient media under conditions of controlled environment has greatly enlarged the scope of experimental investigations. In phytopathology, application of this technique has given an insight into the nature of abnormal growth, factors affecting penetration, infection and multiplication of pathogens, the weapons of attack of the pathogen, and the morphogenetic potential of the diseased cell. The paper reviews recent applications of the technique in studies on parasitism and points some future possibilities.

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References

  • Allen, P. J... “Physiology and biochemistry of defense,”In Horsfall, J. G. and Dimond A. E. Editors,Plant Pathology, Academic Press, New York, 1959,2, 435–67.

    Google Scholar 

  • Arya, H. C... “Cultural behaviour of insect gall and normal plant stem single cell clones,”In C. V. Ramakrishnan, Editor,Tissue Culture (Proc. Seminar Baroda), W. Jank, The Hague, 1965, 293–309.

    Google Scholar 

  • Barker, K. R. and Dailing, H. M. “Reproduction ofAphelenchus avenae on plant tissues in culture,”Nematologica, 1965,11, 162–66.

    Article  Google Scholar 

  • Black, L. M... “Physiology of virus induced tumors in plants,”In Richland W., Editor,Handbuch der Pflanzenphysiologie, Springer, Verlag, Berlin, 1966,15, 246–66.

    Google Scholar 

  • Braun, A. C... “A physiological basis for autonomous growth of the crown-gall tumor cell,”Proc. natn. Acad. Sci., U.S.A., 1958,44, 344–49.

    Article  CAS  Google Scholar 

  • —————.. “A demonstration of the recovery of the crown-gall tumor cell with the use of complex tumors of single cell origin,” Ibid., 1959,45, 932–38.

    Article  CAS  Google Scholar 

  • —————.. “The reversal of tumor growth,”Sci. Amer., 1965,213, 75–83.

    PubMed  CAS  Google Scholar 

  • —— and Lipetz, J... “The use of tissue cultuare in phytopathology,”In Willmer, E. N., Editor,Cells and Tissues in Culture, Academic Press, London, 1966,3, 692–722.

    Google Scholar 

  • ——— and Wood, H. N... “On the activation of certain essential biosynthetic systems in cells ofViacarosea L.,”Proc. natn. Acad. Sci., U.S.A., 1962,48, 1776–82.

    Article  CAS  Google Scholar 

  • Camus, G... “Recherches sur le role des bourgeons dans les phenomenes de morphogénése,”Rev. Cyt. Biol. Vég. 1949,9, 1–191.

    Google Scholar 

  • Chandra, N. and Hildebrandt, A. C. “Growth in microculture of single toba co cells infected with tobacco mosaie virus,”Science, N.Y., 1956,152, 789–891.

    Google Scholar 

  • —— and ——.. “Differentiation of Lunts from tobacco mosaic virus inclusion-bearing and inclusion-free single tobacco cells,”Virology 1967,31, 414–21.

    Article  PubMed  CAS  Google Scholar 

  • Chen, Tseh-An, Kilpatrick, R. A. and Rich, A. E. “Sterile culture techniques as tools in plant nematology research,”Phytopathology, 1961,51, 799–800.

    Google Scholar 

  • Clatter, Mary E... “Hormonal induction of vascular tissue in tobacco pithin vitro,”Science, N.Y., 1960,132, 548–49.

    Google Scholar 

  • Darling, H. M., Faulkner, L. R. and Wallendal, P. “Culturing the potato rot nomatode (Abstr.),”Phytopathology, 1957,47, 7.

    Google Scholar 

  • Das, T. M., Hildebrandt, A. C. and Riker, A. J. “Cinephotomicrography of low temperature effects on cytoplasmic streaming, nucleolar activity and mitosis in single tobacco cells in microculture,”Am. J. Bot., 1966,53, 253–59.

    Article  PubMed  CAS  Google Scholar 

  • Dolliver, J. S., Hildebrandt, A. C. and Riker, A. J. “Studies of reproduction ofAphelencoides ritzemabosi, Schwartz on plant tissues in culture,”Nematologica, 1962,7, 294–300.

    Google Scholar 

  • Dougherty, E. C... “Cultivation of Aschelminths especially rhabditid nematodes,”In Sasser, J. N. and Jenkins, W. R., Editors,Nematology, Fundamentals and Recent Advances with Emphasis on Plant Parasitic and Soil Forms, The University of North Carolina Press, Chapel Hill, 1960, 297–318.

    Google Scholar 

  • Haberlandt, G... “Kulturberusche mit isolierten Pflanzenzellen,”Sitzungsber. Akad. der Wiss. Wien, Math. Naturwiss., 1902,111, 69–29.

    Google Scholar 

  • Hansen, A. J. and Hildebrandt, A. C. “The distribution of tobacco mosaic virus in plant callus cultures,”Virology, 1966,28, 15–21.

    Article  PubMed  CAS  Google Scholar 

  • Helgeson, J. P. and Leonard, N. J. “Cytokinins: identification of compounds isolated fromCorynebacterium fascians,”Proc. natn. Acad. Sci., U.S.A., 1966,56, 60–63.

    Article  CAS  Google Scholar 

  • Hildebrandt, A. C... “Growth of single cell clones of diseased and normal tissue origins,”In Plant Tissue Culture and Morphogenesis, Scholar’s Library, New York, pp. 1963, 1–22

    Google Scholar 

  • —— and Riker, A. J... “Viruses and single cell clones in plant tissue culture,”Fed. Proc., 1958,17, 986–93.

    PubMed  CAS  Google Scholar 

  • Hirth, L. and Lebeurier, G... “Remarques sur la sensibilité des cellules des cultures de tissus de tabac à l’infection par le virus de la mosaique du tobac on son acide ribonucleique,”Rev. gén. Bot., 1965,62, 5–20.

    Google Scholar 

  • Jones, L. E., Hildebrandt, A. C., Riker, A. J. and Wu, J. H. “Growth of somatic tobacco cells in microculture,”Am. J. Bot., 1960,47, 468–75.

    Article  Google Scholar 

  • Kalil, Millicent, Hildebrandt, A. C. and Evert, R. F. “The influence of virulent crown-gall bacteria and non-virulentAgrobacterium radiobacter on starch metabolism in tomato cells in microculture (Abstr.),” Ibid., 1965,52, 633.

    Google Scholar 

  • Kassanis, B., Tinsley, T. W. and Quak, F. “The inoculation of tobacco callus tissue with tobacco mosaic virus,”Ann. appl. Biol., 1958,46, 11–19.

    Article  Google Scholar 

  • Klämbt, D., Thies, Gail and Skoog, F. “Isolation of cytokinins fromCorynebacterium fascians,”Proc. natn. Acad. Sci., U.S.A., 1966,56, 52–59.

    Article  Google Scholar 

  • Krapasagar, V. and Barker, K. R. “Increased cytokinin concentrations in tobacco infected with root-knot nematodeMeloidogyne incognita (Abstr.),”Phytopathology, 1966,56, 885.

    Google Scholar 

  • Krusberg, L. R. and Blickenstaff, M. L. “Influence of plant growth regulating substances on reproduction ofDitylenchus dipsaci, Pratylenchus penetrans andPratylenchus zeae on alfalfa tissue cultures,”Nematologica, 1964,10, 145–50.

    Google Scholar 

  • Letham, D. S... “Chemistry and physiclogy of kinetin-like compounds,”A. Rev. Pl. Physiol., 1967,18, 349–64.

    Article  CAS  Google Scholar 

  • Libbert, E., Wichner, S., Schiewer, U., Risch, H. and Kaiser, W. “The influence of epiphytic bacteria on auxin metabolism,”Planta, 1966,68, 327–34.

    Article  CAS  Google Scholar 

  • Libbert, E., Wichner, S., Kaiser, W., Manicki, A., Manueuffel, R., Riecke, E. and Schröder, R. “Auxin content and anabolism of sterile and non-sterile plants, with special regard to the influence of epiphytic bacteria (Abstr.),”Sixth International Conference on Plant Growth Substances, Carleton University, 1967.

  • Maheshwari, R... “The physiology of penetration and infection by arediospores of rust fungi,”Ph.D. Thesis, University of Wisconsin, Madison, 1966.

    Google Scholar 

  • ——, Allen, P. J. and Hildebrandt, A. C. “Physical and chemical factors controlling the development of infection structures from urediospore germ tubes of rust fungi,”Phytopathology, 1967a,57, 855–62.

    CAS  Google Scholar 

  • ——, Hildebrandt, A. C. and Allen, P. J. “Factors affecting the growth of rust fungi on host tissue cultures,”Bot. Gaz., 1967b,128, 153–59.

    Article  Google Scholar 

  • Matsubara, S. and Nakahira, R. “Cytokinin activity in an extract from the gall ofPlasmodiophora-infected root ofBrassica rapa L.”Bot. Mag. Tokyo, 1967,80, 373–74.

    Google Scholar 

  • Melchers, G. .. “Genetical aspects in callus culture work (Abstr.),” International Symposium on Plant Pathology, New Delhi,Bull. Indian Phytopath. Soc., 1968 (In press).

  • Miller, C. O... “Zeatin and zeatin riboside from a mycorrhizal fungus,”Science, N.Y., 1967,157, 1055–57.

    Google Scholar 

  • Mountain, W. B... “Acceptable standards of proof and approaches for evaluating plant-nematode relationships,”In Sasser, J. N. and Jenkins, W. R., Editors,Nematology, Fuadamentals and Recent Advances with Emphasis on Plant Parasitic and Soil Forms, The University of North Carolina Press, Chapel Hill, 1960, pp. 422–25.

    Google Scholar 

  • Morul, G... “Recherches sur la culture associee de Parasites obligatoires et de tissus vegetaux,”Ann. Epiphyties, 1948,14, 123–234.

    Google Scholar 

  • Muir, W. H., Hildebrandt, A. C. and Riker, A. J. “The preparation, isolation and growth in culture of single cells from higher plants,”Am. J. Bot., 1958,45, 589–97.

    Article  Google Scholar 

  • Nakamura, H... “The use of tissue cultures in the study of obligate parasites,”In White, P. R. and Grove, A. R., Editors,Proc. International Conference on Plant Tissue Culture, McCutchan Publishing Corp., Berkeley, 1965.

    Google Scholar 

  • Pelet, F... “Growthin vitro of grape, elm, poplar, willow and oak tissues isolated from normal stems and insect galls,”Ph.D. Thesis, University of Wisconsin, Madison, 1959.

    Google Scholar 

  • Penso, G. and Balducci, D...Tissue Cultures in Biological Research, Elsevier Publishing Co., Amsterdam, 1963.

    Google Scholar 

  • Raychaudhuri, S. P... “Plant viruses in tissue culture,”Adv. Virus Res., 1966,12, 175–206.

    Article  PubMed  CAS  Google Scholar 

  • —— and Mishra, M. D... “Effect of some metabolites and their analogues on the infectivity of tobacco tissue culture containing chilli mosaic virus,”Virology, 1965,25, 483–84.

    Article  CAS  Google Scholar 

  • Riker, A. J... “Studies on the influence of some environmental factors on the development of crown-gall,”J. agric. Res., 1926,57, 545–55.

    Google Scholar 

  • Sandstedt, R. and Schuster, M. L. “Excised tobacco pith bioassays for root-knot nematodeproduced plant growth substances,”Physiol. Plant., 1966a,19, 99–104.

    Article  CAS  Google Scholar 

  • —— and ——.. “The role of auxins in root-knot nematode-induced growth on excised tobacco stem segments,” Ibid., 1966b,19, 960–67.

    Article  CAS  Google Scholar 

  • Sayre, R. M... “Plant tissue culture as a tool in the study of the physiology of root-knot nematodeMeloidogyne incognita Chit,”Ph.D. Thesis, University of Nebraska, Lincoln, 1958.

    Google Scholar 

  • Schroeder, P. H. and Jenkins, W. R. “Reproduction ofPratylenchus penetrans on root tissues grown on three media,”Nematologica, 1963,9, 327–31.

    Article  Google Scholar 

  • Schuster, M. L. and Sullivan, T. “Species differentiation of nematodes through host reaction in tissue culture. I. Comparisons ofMeloidogyne hapla, M. incognita andNacobbus batatiformis,”Phytopathology, 1960,50, 874–75.

    Google Scholar 

  • Singh, H... “The growth ofAlbugo in the callus culture ofIpomoea,”Curr. Sci., 1963,32, 472–73.

    Google Scholar 

  • —————.. “On the variability of the callus ofIpomoea-infected withAlbugo grown underin vitro conditions,”Phytomorphology, 1966,16, 189–92.

    Google Scholar 

  • Singh, M. and Hildebrandt, A. C. “Movements of tobacco mosaic virus inclusion bodies within tobacco callus cells,”Virology, 1966,30, 134–42.

    Article  PubMed  CAS  Google Scholar 

  • Steward, F. C., Kent, Ann E., and Mapes, Marion O. “The culture of free plant cells and its significance for embryology and morphogenesis,”In Monroy, A. and Moscona, A. A., Editors,Current Topics in Developmental Biology, Academic Press, New York, 1966, pp. 113–54.

    Chapter  Google Scholar 

  • Strandberg, J. O., Williams, P. H. and Yorkawa, Y. “Monoxenic culture ofPlasmodiophora brassicae with cabbage tissue (Abstr.),”Phytopathology, 1966,56, 903.

    Google Scholar 

  • Thimann, K. V. and Sachs, T. “The role of cytokinins in the ‘fasciation’ disease caused byCoryncbacterium fascians,”Am. J. Bot., 1966,53, 731–39.

    Article  CAS  Google Scholar 

  • --, Grochowska, M. and Avadhani, P. N. “The role of tryptophan as an I.A.A. precursor (Abstr.),”Sixth International Conference on Plant Growth Substances, Carleton University, 1967.

  • Vasil, Vimla and Hildebrandt, A. C. “Differentiation of tobacco plants from single, isolated cells in microculture,”Science, N.Y., 1965,150, 889–92.

    CAS  Google Scholar 

  • —— and —— “Further studies on the growth and differentiation of single isolated cells of tobaccoin vitro,”Planta, 1967,75, 139–51.

    Article  Google Scholar 

  • Warick, R. P. and Hildebrandt, A. C. “Free amino-acid contents of stem andPhylloxera gall tissue cultures of grape,”Pl. Physiol., 1966,41, 573–78.

    Article  CAS  Google Scholar 

  • Webster, J. M... “Production of oat callus and its susceptibility to a plant parasitic nematode,”Nature, Lond., 1966,212, 1472.

    Article  CAS  Google Scholar 

  • —— and Lowe, D... “The effect of the synthetic plant-growth substance, 2,4-dichlorophenoxyacetic acid, on the host-parasite relationship of some plant parasitic nematode in monaxenic culture,”Parasitology, 1966,56, 312–22.

    Article  Google Scholar 

  • Wetmore, R. H. and Rier, J. P. “Experimental Induction of vascular tissues in callus of angiosperms,”Am. J. Bot., 1953,50, 418–30.

    Article  Google Scholar 

  • White, P. R... “Versatile perfusion chamber for living cells and organs,”Science, N.Y., 1966,152, 1758–60.

    CAS  Google Scholar 

  • —— and Braun, A. C. “A cancerous neoplasm of plants: Autonomous bacteria-free crown-gall tissue,”Cancer Res., 1942,2, 597–617.

    Google Scholar 

  • Williams, P. H. and Yukawa, Y. B. “Ultrauturctural studies on the host-parasite relations ofPlasmodiophora brassicae,”Phytopathology, 1967,57, 682–87.

    Google Scholar 

  • Wood, H. N. and Braun, A. C. “Studies on the regulation of certain essential biosynthetic systems in normal and crown-gall tumor cell,”Proc. natn. Acad. Sci., U.S.A., 1961,47, 1907–13.

    Article  CAS  Google Scholar 

  • —— and ——.. “Studies on the net uptake of solutes by normal and crown-gall tumor cells,”Proc. natn. Acad. Sci., U.S.A., 1965,54, 1532–38.

    Article  CAS  Google Scholar 

  • Zachau, H. G., Dutting, D. and Feldman, H. “Nucleotide sequence of two serine-specific transfer ribonucleic acid,”Angew. Chem. Internat. Edit., 1966,5, 422.

    Article  CAS  Google Scholar 

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  1. Department of Botany, University of Delhi, Delhi-7

    Ramesh Maheshwari

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Maheshwari, R. Applications of plant tissue and cell culture in the study of physiology of parasitism. Proc. Indian Acad. Sci. 69, 152–172 (1969). https://doi.org/10.1007/BF03052523

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