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Studies on the nucleolus

I. The nucleolar membrane of rat liver cells

  • Published:
Proceedings / Indian Academy of Sciences

Summary

Evidence for the presence of a nucleolar membrane in rat liver cells is marshalled from a variety of techniques. The basophily of the nucleolar matrix has to be removed in order to locate this membrane. Unlike acetocarmine and hæmatoxylin, Feulgen enabled the specific identification of chromatin but not the nucleolar membrane. Under specified conditions, the nuclear and nucleolar membranes could be seen in squashes of hydrolysed cells stained with acridine orange (1:40,000) and examined under u.v. illumination. Convincing proof for the presence of a nucleolar membrane was obtained by examination under phase contrast, cells, either hydrolysed in N HCl at 60° C. for 5 min.; or lightly tinted with acridine orange, after exposure to 45% acetic acid at 60° C. for 2–5 min.

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Authors and Affiliations

  1. Scientists’ Pool of C.S.I.R., Cytogenetics Laboratory, Department of Biochemistry, Indian Institute of Science, Bangalore-12

    Saraswathy Royan

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  1. Saraswathy Royan
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Communicated by Dr. M. K. Subramaniam,f.a.sc.

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Royan, S. Studies on the nucleolus. Proc. Indian Acad. Sci. 55, 201–212 (1962). https://doi.org/10.1007/BF03052321

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  • DOI: https://doi.org/10.1007/BF03052321

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