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Amino-acids and sugars in the exudates from germinating seeds of rice

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Proceedings / Indian Academy of Sciences

Abstract

Substances exuding from rice seeds (Oryza sativa L.) allowed to germinate under aseptic and non-aseptic conditions were examined at different stages during germination (0–120 hrs.). Several strains of indica and japonica rice,viz., Co13, Ptb1, Mtu9, Taiwan 3, TKM6, IR8 and and Adt27 were examined on this count. Leucine, glutamic acid, valine and alanine constituted more than 80% of the total amino-acids exuded out. The three pure line indicas gave out the maximum amounts of amino-acids. Considerable differences in the amino-acid and sugar contents in the exudates were noticed when the seeds were surface-sterilized with mercuric chloride (0·2%). Exudates from surface-sterilized seeds always were richer than the exudates from untreated seeds except in the initial stages. The content of amino-acid and sugar in the former increased with time upto a certain period while there was a progressive decrease in the latter.

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References

  • Agnihotri, V. P. and Vaartaja, O. “Root exudates from red pine seedlings and their effect onPythium ultimum,”Can. J. Bot., 1967,45, 1030–40.

    Google Scholar 

  • Block, R. J., Durram, E. L. and Zweig, G.A Manual of Paper Chromatography and Paper Electrophoresis, Academic Press, New York, 1963, pp. 18–126.

    Google Scholar 

  • Börner, H... “Liberation of organic substances from higher plants and their role in the soil sickness problem,”Bot. Rev., 1960,26, 393–424.

    Article  Google Scholar 

  • Cook, R. J. and Flentje, N. T... “Chlamydospore germination and germling survival ofFusarium solani f.pisi in soil as affected by soil water and pea seed exudation,”Phytopathology, 1967,57, 178–82.

    Google Scholar 

  • —— and Snyder, W. C... “Influence of host exudate on growth and survival of germlings ofFusarium solani f.phaseoli in soil,” Ibid., 1965,55, 1021–25.

    Google Scholar 

  • Flentje, N. T... “Pre-emergence rotting of peas in South Australia. II. Factors associated with soil,”Aust. J. biol. sci., 1964,17, 651–64.

    Google Scholar 

  • —— and Saksena, M. K... “Pre-emergence rotting of peas in South Australia. III. Host pathogen interaction,” Ibid., 1964,17, 655–75.

    Google Scholar 

  • Juliano, B. O. .. “Physicochemical studies of rice starch and protein,”International Rice Commission Newsletter, Special Issue, 1967.

  • Kerr, A... “The influence of soil moisture on infection of peas byPythium ultimum,”Aust. J. biol. Sci., 1964,17, 676–85.

    Google Scholar 

  • Müller, H... “Untersuchungen zur Frage wechselseitiger Bezuhungen Zwischen keimenden zamen und Mikro-organismon im samennähe,”Arch. Microbiol., 1962,41, 351–82.

    Google Scholar 

  • Nelson N... “A photometric adaptation of the Somogyi method for the determination of glucose,”J. biol. Chem., 1944,153, 375–80.

    CAS  Google Scholar 

  • Pearson, R. and Parkinson, D. “The sites of excretion of ninhydrin positive substances by broad bean seedlings,”Pl. Soil, 1961,13, 391–96.

    Article  CAS  Google Scholar 

  • Porter, C. A. Margolis, D. and Sharp, P. “Quantitative determination of amino-acids by paper chromatography,”Contr. Boyce Thompson Inst. Pl. Res., 1954,18, 465.

    Google Scholar 

  • Rajagopalan, K. and Bhuvaneswari, K... “Effect of germination of seeds and host exudation during germination on foot rot disease of rice.Phytopath. Z., 1964,50, 221–26.

    Article  Google Scholar 

  • Richardson, L. T... “Reversal of fungitoxicity of thiram (toGlomerella cingulata) by (soybean) seed and root exudates,”Can. J. Bot., 1966,44, 111–12.

    Article  CAS  Google Scholar 

  • Schroth, M. N. and Cook, R. J. “Seed exudation and its influence on pre-emergence damping-off of bean,”Phytopathology, 1964,54, 670–73.

    Google Scholar 

  • —— and Hildebrand, D. C... “Influence of plant exudates on root infecting fungi,”A. Rev. Phytopathol., 1964,2, 101–32.

    Article  Google Scholar 

  • —— and Snyder, W. C... “Effect of host exudates of chlamydospore germination of the bean root rot fungusFusarium solani f.phaseoli,”Phytopathology, 1961,51, 389–93.

    CAS  Google Scholar 

  • Schroth, M. N., Toussoun, T. A. and Snyder, W. C. “Effect of certain constituents of bean exudate on germination of chlamydospores ofFusarium solani f.phaseoli in soil,” Ibid., 1963,53, 803–12.

    Google Scholar 

  • ——, Weinhold, A. R. and Hayman, D. S. “The effect of temperature on quantitative differences in exudates from germinating seeds of bean, pea and cotton,”Can. J. Microbiol., 1966,44, 1429–32.

    Google Scholar 

  • Shanthi, B. .. “Physiology of spore germination in fungi,”Doctoral Thesis, Univ. Madras, 1967.

  • Shaw, M. and Colotelo, N. “The physiology of host parasite relations. VII. The effect of stem rust on the nitrogen and amino-acid in wheat leaves,”Can. J. Bot., 1961,39, 1351.

    Article  CAS  Google Scholar 

  • Singh, R. S. “Development ofPythium ultimum in soil in relation to presence and germination of seeds of different crops,”Mycopath. Mycol. appl., 1965,27, 155–60.

    Article  CAS  Google Scholar 

  • Sircar, S. M. “Physiology of the rice plant,” Presidential Address. Section of Botany,Indian Science Congress, Delhi, 1963.

  • ————— “Biochemical changes of rice seed germination and its control. mechanisms,”Trans. Bose Res. Inst., 1967a,30, 189–98.

    CAS  Google Scholar 

  • ----- .. “Biochemical changes during germination of rice seeds,” InPhysiology, Ecology and Biochemistry of Germination, Greenswald, Ernst-Moritz-Arndt-Universitat, 1967b, pp. 489–94.

  • —— and Dastidar, A. G. “Amino-acid metabolism of the seeds of rice during germination and seedling growth,”Physiologia Pl., 1962,15, 206–10.

    Article  CAS  Google Scholar 

  • Sircar, S. M. and Ray, P. D. “Studies in the physiology of rice. XV. Changes in the metabolism of the seed during germination and their relationship to the application of growth regulators,”J. expt. Bot., 1962,13, 61–74.

    Article  CAS  Google Scholar 

  • Somogyi, M... “Notes on sugar determination,”J. biol. Chem., 1952,195, 19–23.

    CAS  Google Scholar 

  • Stepka, W... “Identification of amino-acids by paper chromatography,” InMethods in Enzymology, Vol. III, Editors Colowick, S. P. and Kapalan, N.O., Acad. Press, New York, 1957, pp. 504–28.

    Google Scholar 

  • Wang, D... “Metabolism of amino-acids and amides in germinating seeds,”Contr. Boyce Thompson Inst. Pl. Res., 1968,24, 109–15.

    CAS  Google Scholar 

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Authors and Affiliations

  1. University Botany Laboratory, Madras-5

    K. M. Leelavathy

Authors
  1. K. M. Leelavathy
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Communicated by Prof. T. S. Sadasivan,f.a.sc.

Memoir No. 79 from the Centre for Advanced Studies in Botany.

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Leelavathy, K.M. Amino-acids and sugars in the exudates from germinating seeds of rice. Proc. Indian Acad. Sci. 72, 81–90 (1970). https://doi.org/10.1007/BF03051742

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  • DOI: https://doi.org/10.1007/BF03051742

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