Abstract
A potent and specific antiserum was raised in rabbits by injecting a single cell suspension of thymocytes of the lizard,Calotes versicolor. Rabbit anti-Calotes thymocyte serum (ATS) was characterizedin vitro by using cytotoxicity assay and quantitative absorption analysis. The cytolytic activity of ATS was always higher towards thymocytes than towards lymphoid cells of spleen, bone-marrow and peripheral blood. Various absorption experiments indicate the existence of two antigens among thymocytes—one distinct for thymocytes and thymus derived cells and another, common to lymphocytes. Further, thymus-brain antigenic correlation has been demonstrated in this reptilian species. TheCalotes complement is less efficient than that of guinea pig in mediating the cytolysis of target cells by ATS. Further, ATS did not have cytotoxic effect on antibody producing cells. The significance of these findings has been discussed with reference to the dichotomy of lymphoid systems at this phylogenetic level.
Similar content being viewed by others
References
Turk, J. L. and Willoughby, D. A.,Lancet i 249 (1967).
Martin, W. J. and Miller, J. F. A. P.,Lancet ii 1285 (1967).
Potworowski, E. F., Richer, G., Borduas, A. and Forget, A.,J. Immunol. 106 1416 (1971).
Kerbel, R. S. and Eidinger D.,J. Immunol. 106 917 (1971).
Diener, E., Collaghan, F. D. and Kraft, N.,J. Immunol. 107 1775 (1971).
Raff, M. C. and Owen, J. J. T.,Eur. J. Immunol. 1 27 (1971).
Toben, H. R. and Pierre, R. S. St.,Int. Arch. Allergy Appl. Immunol. 42 454 (1972).
Lance, E. M., Medawar, P. B. and Taub, R. N.,Adv. Immunol. 1 (1973).
Cohen, N.,J. Am. Vet. Med. Assoc. 159 1662 (1971).
Du Pasquier, L., inCurrent Topics in Microbiology and Immunology 61 38 (1973).
Manickavel, V. and Muthukkaruppan, VR.,Transplantation 8 307 (1969).
Muthukkaruppan, VR., Kanakambika, P., Manickavel, V. and Veeraraghavan, K.,J. Morphol. 130 479 (1970).
Kanakambika, P., Doctoral thesis submitted to Annamalai University, India (1971).
Manickavel, V., Doctoral thesis submitted to Annamalai University, India (1972).
Kanakambika, P. and Muthukkaruppan, VR.,Proc. Soc. Exp. Biol. Med. 140 21 (1972).
Kanakambika, P. and Muthukkaruppan, VR.,J. Immunol. 109 415 (1972).
Kanakambika, P. and Muthukkaruppan, VR.,Experientia 28 1225 (1972).
Kanakambika, P. and Muthukkaruppan, VR.,Proc. Indian Acad. Sci. B 78 37 (1973).
Pitchappan, RM., Doctoral thesis submitted to Madurai University, India (1975).
Jayaraman, S. and Muthukkaruppan, VR.,Experientia (in press) (1975).
Muthukkaruppan, VR. Pitchappan, RM. and Ramila, G.,Amer Zool. (in press) (1976).
Muthukkaruppan, VR. Subramania Pillai, P. and Jayaraman, S., In Cleveland clinic symposium (1976).
Pitchappan, RM. and Muthukkaruppan, VR.,Proc. Indian Acad. Sci. B 84 42 (1976).
Gozzo, J. J., Wood, M. L. and Monaco, A. T.Transplantation 14 352 (1972).
Saijo, N.Immunology 24 683 (1973).
Zimpel, H., Stark, R. and Thiele, H. G.,J. Immunol. Methods 3 411 (1973).
Cunningham, A. J. and Szenberg, A.,Immunology 14 599 (1968).
Wick, G., Albini, B. and Milgrom, F.,Clin. exp. Immunol. 15 237 (1973).
Reif, A. E., and Allen, J. M. V.,Nature London 209 521 (1966).
Raff, M. C. and Wortis, H. H.,Immunology 18 931 (1970).
Colley, D. G., Malkian, A. and Waksman, B. H.J. Immunol. 104 585 (1970).
de la Noue, H., Koperstych, S. and Richter, M.,Immunology 23 655 (1972).
Reif, A. E. and Allen, J. M. V.,Nature London 209 523 (1966).
Scheid, M., Boyse, E. A., Carswell, E. A. and Old, L. J.,J. Exp. Med. 135 938 (1972).
Michael, B., Pasternak, G. and Steuden, J.,Nature London New Biol. 241 221 (1973).
Thiele, H. G., Stark, R., Keeser, D. and Zimpal. H.,Z. Immunol. Forsch. 146 346 (1974).
Raff, M. C. and Cantor, H.,Progress in Immunology, ed. B. Amos (Academic Press, New York), p. 83 (1971).
Nossal, G. J. V., Cunningham, A., Mitchell, G. F. and Miller, J. F. A. P.,J. Exp. Med. 128 839 (1968).
Kindred, B.,J. Immunol. 107 1291 (1971).
Barth, R. F., and Carroll, G. F.,J. Immunol. 104 522 (1970).
Cinader, B., Jeejeebhoy, H. F., Koh, S. W. and Rabbat, A. G.,J. Exp. Med. 133 81 (1971).
Author information
Authors and Affiliations
Additional information
Supported by a grant from U.S. National Institutes of Health (01-077), under special foreign currency research programme (PL-480).
Rights and permissions
About this article
Cite this article
Pitchappan, R., Muthukkaruppan, V. In vitro properties of heterologous anti-lizard thymocyte serum. Proc. Indian Acad. Sci. 85, 1–12 (1977). https://doi.org/10.1007/BF03050887
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03050887