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Differential fluorescence of the chromocenters and nucleolar equivalents of the yeast nucleus in acridine orange

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Proceedings / Indian Academy of Sciences

Summary

The nucleus with its limiting membrane and organelles was visible in the majority of the yeast cells stained vitally with the fluorochrome, acridine orange, at a dilution of 1 in 40,000. The intra-nuclear structures could be distinguished by their differential fluorescence. The chromocenters were green while the nucleolar equivalents were orange. The vacuole showed no fluorescence.

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Authors and Affiliations

  1. Cytogenetics Laboratory, Department of Biochemistry, Indian Institute of Science, Bangalore-12

    Saraswathy Royan & M. K. Subramaniam F.A.Sc.

Authors
  1. Saraswathy Royan
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  2. M. K. Subramaniam F.A.Sc.
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Royan, S., Subramaniam, M.K. Differential fluorescence of the chromocenters and nucleolar equivalents of the yeast nucleus in acridine orange. Proc. Indian Acad. Sci. 51, 205–210 (1960). https://doi.org/10.1007/BF03050027

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  • DOI: https://doi.org/10.1007/BF03050027

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