Abstract
The effects of application of Pan Parag with tobacco on buccal mucosa of 21 albino rats (Wistar strain) were studied. The paste made up of pan Parag (instant betel nut preparation with tobacco) was painted over the entire buccal mocosa of the experimental group (n=21) on every alternate day from the 46th to 255th day of the study with a total of 42 days intervening rest period. Whereas in the control group (N=14), the painting brush dipped in tap water was rubbed over the buccal mucosa in the same frequency. Punch biopsies were taken from the buccal mucosa of all the animals on the 31st day, 106th day, 181st day and 256th day of the study. The animals were left untouched for 15 days following each biopsy procedure. The biopsy specimens in the experimental group after 2 months painting revealed changes of Papillomatosis in 22% and dysplasia in 39% of rats. Dysplastic changes were observed in 83% of the animals at the end of 4th months, which increased to 95% after 6 months. The control group revealed no significant pathological changes. Our study shows that the application of Instant betel nut preparation with tobacco (Pan Parag) causes significant histopathological changes in a short period of time and are responsible for the production of the various premalignant lesions affecting the oral cavity.
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Sinha, B.K., Mann, S.B.S., Mehra, Y.N. et al. Effect of pan masala with tobacco in buccal mucosa of albino rats. IJO & HNS 46, 67–71 (1994). https://doi.org/10.1007/BF03048874
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DOI: https://doi.org/10.1007/BF03048874