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Steroids in the interrenal and gonads of some amphibians andCalotes—A chromatographic study

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Proceedings / Indian Academy of Sciences

Abstract

The gonadal and interrenal steroid ofRana hexadactyla, Rana cyanophlyctis, Rhacophorus maculatus andCalotes versicolor have been analysed by paper chromatography. In the interrenal of both the sexes of three species of frogs studied, aldosterone and corticosterone could be detected. In the testes of all the three species of frogs studied, testosterone was significantly absent; progesterone and 17β estradiol were detected. In the ovary 17β estradiol. estriol and progesterone were identified. In the adrenals ofCalotes, as in frogs, corticosterone and aldosterone were noticed. In the testis testosterone and 17β estradiol were detected; in the ovary progesterone, estriol, estrone and 17β estradiol were found. The significance of the presence of testosterone and the absence of progesterone in the testes is discussed in the light of biosynthetic pathway known in other forms.

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References

  • Arai, R. Shikita, M. and Tamaoki, B. “In vitro bioconversion of 4-C14 to 17 hydroxy progesterone androst-4-ene-3, 17 dione in testicular tissues ofTribolodon hakonesis Gunter,”Gen. Comp. Endocrinol, 1964,4, 68–73.

    Article  CAS  Google Scholar 

  • Barton, G. M., Evans, R. S. and Gardner, J. A. F. “Paper chromatography of phenolic substances,”Nature, 1952,170, 249–50.

    Article  CAS  Google Scholar 

  • Burton, R. B., Zaffaroni, A. and Keutmann, E. H. “Paper chromatography of steroids. II. Corticosteroids and related compounds,”J. Biol. Chem., 1951,188, 763–71.

    PubMed  CAS  Google Scholar 

  • Carstensen, H. Burgers, A. C. J. and Li, C. H. “Demonstration of aldosterone and corticosterone as the principal steroids formed in incubates of adrenals of the American Bull frog (Rana catesbeiana) and stimulation of their production by mammalian adrenocorticotrophin,”Gen. Comp. Endocrinol, 1961,1, 37–50.

    Article  PubMed  CAS  Google Scholar 

  • Chieffi, G... “La lutogenesi nei selaci ovo vivipari. Richerche istolgichee isto chimiche inTorpedo marmorata eTorpedo ocellata,”Publ. Staz. Zool., Napoli, 1961,32, 145–66.

    CAS  Google Scholar 

  • ——.. “Endocrine aspects of reproduction in elasmobranch fishes,”Gen. Comp. Endocrinol., Suppl., 1962,1, 275–85.

    Article  PubMed  CAS  Google Scholar 

  • —— and Lupo, C... “Identification of oestradiol 17β, testosterone and its precursors fromScylliorhinus stellaris testes,”Nature, 1961a,190, 168–70.

    Article  Google Scholar 

  • ——.. “Identificatione degli ormoni steroidi, nei testicoli e negli organi di bidder diBufo vulgaris,”Atti. Accad. Nazl. lincei., 1961b, Ser. 8.30, 399–402.

    CAS  Google Scholar 

  • ——.. “Identification of sex hormone in the ovarian extracts ofTorpedo marmorata andBufo vulgaris,”Gen. Comp. Endocrinol., 1963,3, 149–52.

    Article  PubMed  CAS  Google Scholar 

  • Dale, E... “Steroid extraction by larval frogs,” Ibid., 1962,2, 171–76.

    Article  PubMed  CAS  Google Scholar 

  • —— and Dorfman, R. I... “Conversion of progesterone-4-C14 to testosterone by testicular tissue of the American Bull frog.” Ibid., 1967,9, 313–18.

    Article  PubMed  CAS  Google Scholar 

  • Dellacorte, F, and Cosenza, L. “Sur la presence ‘d’ hormones steroides dans les testicules deTriturus critsatus carnifex (Laur) (Abstr. 33)” Ibid., 1965,5, 679–80.

    Google Scholar 

  • Gallien, L. and Chalumeau-Le-Foulgoc, M. T. “Mise en evidence de steroides oestrogenes dans L’ Juvenile dexenopus laevis. Daudid et cycle des oestrogenes au, cours de la ponte,”C.R. Acad. Sci., 1960,251, 460–62.

    CAS  Google Scholar 

  • Gist, D. H. and De Roos, R. “In vitro production of corticoids by the adrenal of the alligator (Alligator mississipensis),”Am. Zoologist, 1964,4, 394.

    Google Scholar 

  • Gottfried, H. Huang, D. P. Lofts, B. Phillps, J. G. and Tam, W. “In vitro production of steroids by the adrenal and testicular tissues of the Cobra, (Naja naja)”Gen. Comp. Endocrinol., 1967,8, 18–31.

    Article  CAS  Google Scholar 

  • Ozon, R... “Isolement et identification de steoides chez les vertebres inferieurs et les oiseaux,”Annl. Biol. Anim. Biochem. Biophys., 1966,6(4), 537–51.

    Article  CAS  Google Scholar 

  • Simpson, T. H., Wright, R. S. and Hunt, S. U. “Steroid biosynthesis in the testes of dog fish,”J. Endocrinol., 1964,31, 29–38.

    Article  PubMed  CAS  Google Scholar 

  • Slaunwhite, W. R. Jr. and Samuels, L. T. “Progresterone as a precursor of testicular androgens,” J.Biol. Chem., 1956,220, 341–51.

    PubMed  CAS  Google Scholar 

  • Zaffaroni, A., Burton, R. B. and Keutmann, E. H. “Adrenal Cortical hormones—Analysis of Paper partition Chromatography and occurrence in the urine of normal persons,”Science, 1950,111, 6–8.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Jawaharlal Institute of Postgraduate Medical Education and Research, 605006, Pondicherry, India

    S. Kasinathan & S. L. Basu

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  1. S. Kasinathan
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  2. S. L. Basu
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Communicated by Professor R. V. Seshaiya,f.a.sc.

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Kasinathan, S., Basu, S.L. Steroids in the interrenal and gonads of some amphibians andCalotes—A chromatographic study. Proc. Indian Acad. Sci. 77, 190–195 (1973). https://doi.org/10.1007/BF03045390

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  • DOI: https://doi.org/10.1007/BF03045390

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