Abstract
Gaschromatography with electron capture detection belongs to the most important methods for analysing the A-trichothecenes T-2 toxin, HT-2 toxin and diacetoxyscirpenol. The need of a derivatisation for these mycotoxins prior detection is the main disadvantage of this method. In this paper a sensitive high performance liquid chromatography method to analyse the A-trichothecenes in grain is described. The clean up was performed with multifunctional columns (MycoSepTM, Romer Labs, Inc., MO). The detection limits of these methods are 3.5 μg/kg for T-2 toxin, 6.1 μg/kg for HT-2 toxin and 4.1 μg/kg for diacetoxyscirpenol. Limits of quantification were 12.7 μg/kg for T-2, 21.9 μg/kg for HT-2 and 14.8 μg/kg for DAS, respectively recovery rates were about 100% for each compound.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rafai P, Bata A, Vanyi A, Papp Z, Brydl E, Jakab L, Tuboly S, and Tury E (1995) Effect of various levels of T-2 toxin on the clinical staurs, performance and metabolism of growing pigs. The Veterinary Record 136: 485–489.
Prelusky D B, Rotter B B, and Rotter R G (1994) Chapter 9: Toxiclogy of Mycotoxins. In: Mycotoxins in Grain, Miller J D, Trenholm H L (ed), Eagan Press, St. Paul.
Ueno Y (1983) Trichothecenes-Chemical, Bioligical and Toxicological Aspects. Developments in food science 4, Elsevier, Amsterdam.
Kotal R, Holadova K, Hajslova J, Poustka J, and Radova Z (1999) Determination of trichothecenes in cereals. J. of Chromatography A 830: 219–225.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thimm, N., Handl, J. & Binder, M. HPLC-MS-Method for A-Trichothecenes. Mycotox Res 17 (Suppl 2), 206–209 (2001). https://doi.org/10.1007/BF03036437
Issue Date:
DOI: https://doi.org/10.1007/BF03036437