Skip to main content
SpringerLink
Log in

pS2—A new cytosolic protein recognized by monoclonal antibodies as a marker of hormone sensitivity in breast cancer

  • Published:
Cell Biophysics Aims and scope Submit manuscript

Abstract

Using a new immunoradiometric assay (ELSA pS2 Cis-France), a total of 200 cytosols obtained from primary breast tumors were examined for pS2 content, which is an estrogen-regulated protein actually studied as a marker of hormone sensitivity and favorable prognostic factor in breast cancer. In our patient group, the median pS2 value corresponding to 5.3 ng/mg of cytosolic proteins was used as cutoff. pS2 content was not related to menopause status, tumor size, or nodal involvement, whereas a positive correlation was found between pS2 and ER/PgR status. Moreover, the association of pS2 with steroid receptors seems to identify subgroups of patients better than ER/PgR alone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jensen, E. V. (1981) Hormone dependency of breast cancer.Cancer 47, 2319–2326.

    Article  PubMed  CAS  Google Scholar 

  2. McGuire, W. L. (1988) Hormone, receptors and breast cancer.Prog. Cancer Res. Ther. 10, 1–42.

    Google Scholar 

  3. Osborne, C. K., Yochinowitz, M. G., Knight, W. A., and McGuire, W. L. (1980) The value of estrogen and progesterone receptors in the treatment of breast cancer.Cancer 46, 2884–2888.

    Article  PubMed  CAS  Google Scholar 

  4. Dickson, R. B. and Lipman, M. E. (1987) Estrogenic regulation of growth and polypeptide growth factor secretion in human breast carcinoma.Endocr. Rev. 8, 29–43.

    Article  PubMed  CAS  Google Scholar 

  5. Rio, M. C. and Chambon, P. (1990) The pS2 gene, mRNA and protein: a potential marker for human breast cancer cell line (review).Cancer Cells 2, 269–273.

    PubMed  CAS  Google Scholar 

  6. Masiakowski, P., Breathnach, R., Bloch, J., Gannon, F., Krust, A., and Chambon, P. (1982) Cloning of c-DNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line.Nucleic Acid Res.10, 7895–7903.

    Article  PubMed  CAS  Google Scholar 

  7. Nunez, A. M., Jakowlev, S., Briand, J. P., Gaire, M., Krust, A., Rio, M. C., and Chambon, P. (1987) Characterization of the estrogen induced pS2 protein secreted by the human breast cancer cell line MCF-7.Endocrinology 121, 1759–1765.

    PubMed  CAS  Google Scholar 

  8. Henry, J. A., Piggott, N. H., Mallick, U. K., Nicholson, S., Farndon, J. R., Westley, B. R., and May, F. E. B. (1991) pNR-2/pS2 immunohistochemical staining in breast cancer: correlation with prognostic factors and endocrine response.Br. J. Cancer 63, 615–622.

    PubMed  CAS  Google Scholar 

  9. Jakowlew, S. B., Breathnach, R., Jetsch, J. M., Masiakowski, P., and Chambon, P. (1984) Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7.Nucleic Acid Res.12, 2861–2878.

    Article  PubMed  CAS  Google Scholar 

  10. Berry, M., Nunez, A. M., and Chambon, P. (1989) Estrogen-responsive element of the human pS2 gene is an imperfectly palindromic sequence.Proc. Natl. Acad. Sci. USA 86, 1218–1222.

    Article  PubMed  CAS  Google Scholar 

  11. Rio, M. C., Lepage, P., Diemunsch, P., Roitsch, C., and Chambon, P. (1988) Structure primaire de la proteine humaine pS2.CR Acad. Sci. Paris 307, 825–831.

    CAS  Google Scholar 

  12. Rio, M. C., Bellocq, J. P., Daniel, J. Y., Tomasetto, C., Lathe, R., Chenard, M. P., Batzenschlager, A., and Chambon, P. (1988) Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa.Science 241, 705–708.

    Article  PubMed  CAS  Google Scholar 

  13. Baker, M. E. (1988) Estrogen-induced pS2 protein is similar to pancreatic spasmolitic polypeptide and the kringle domain.Biochem. J. 155, 366–372.

    Google Scholar 

  14. Henry, J. A., Bennett, M. K., Piggott, N. H., Levett, D., May, F. E. B., and Westley, B. R. (1991) Expression of the pNR/pS2 protein in diverse human epithelial tumors.Br. J. Cancer 64, 677–682.

    PubMed  CAS  Google Scholar 

  15. Schwartz, L. H., Koerner, F. C., Edgerton, S. M., Sawicka, J. M., Rio, M. C., Bellocq, J. P., Chambon, P., and Thor, A. D. (1991) pS2 expression and response to hormonal therapy in patients with advanced breast cancer.Cancer Res. 51, 624–628.

    PubMed  CAS  Google Scholar 

  16. Predine, J., Spyratos, F., and Prud’Homme, J. F. (1992) Enzyme-linked immunosorbent assay of pS2 in breast cancers, benign tumors, and normal breast tissues: correlation with prognosis and adjuvant hormone therapy.Cancer 69, 2116–2123.

    Article  PubMed  CAS  Google Scholar 

  17. Rio, M. C., Bellocq, J. P., Geirard, B., Rasmussen, V. B., Krust, A., Kochl, C., Calderoli, H., Schiff, V., Renoud R., and Chambon, P. (1987) Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERB-B2.Proc. Natl. Acad. Sci. USA 241, 9243–9247.

    Article  Google Scholar 

  18. Foekens, J. A Rio, M. C., Seguin, P., Van Putten, W. L. J., Faugue, J., Nap, M., Klijn, J. G. M., and Chambon, P. (1990) Prediction of relapse and survival in breast cancer patients by pS2 protein status.Cancer Res. 50, 3832–3837.

    PubMed  CAS  Google Scholar 

  19. McGuire, W. L. and De La Garza, M. (1973) Improved sensitivity in the measurement of estrogen receptor in human breast cancer.J. Clin. Endocr. 37, 986–989.

    PubMed  CAS  Google Scholar 

  20. Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding.Analyt. Biochem. 72, 248–253.

    Article  PubMed  CAS  Google Scholar 

  21. Piffanelli, A., Pelizzola, D., Giovannini G., Catozzi, L., Faggioli, L., and Giganti, M. (1989) Characterization of laboratory working standards for quality control of immunometric and radiometric estrogen receptor assays. Clinical evaluation on breast cancer biopsies.Tumori 75, 550–556.

    PubMed  CAS  Google Scholar 

  22. May, F. E. B. and Westley, B. R. (1988) Identification and characterization of estrogen regulated RNAs in human breast cancer cells.J. Biol. Chem. 263, 12,901–12,908.

    CAS  Google Scholar 

  23. Westley, B. R., Holzel, F., and May F. E. B. (1989) Effects of oestrogen and antioestrogens, tamoxifen and LY 117018, on four oestrogen regulated RNAs in EFM-19 breast cancer cell line.J. Steroid Biochem. 32, 365–372.

    Article  PubMed  CAS  Google Scholar 

  24. Correale, M., Paradiso, A., Abbate, I., Mangia, A., Dragone, C. D., Musci, M. D., Filotico, R., Schittulli, F., and De Lena, M. (1993) Cytosolic levels of estrogen-regulated pS2 protein in breast cancer: correlation with tumor proliferative activity.Tumor Biol. 14, 30–37.

    Article  CAS  Google Scholar 

  25. Zaretsky, J. Z., Weiss, M., Tsakfaty, I., Hareuveni, M., Wreschner, D. H., and Keydar, I. (1990) Expression of genes coding for pS2, c-erb B2, estrogen receptor and the H23 breast tumor-associated antigen: a comparative analysis in breast cancer.FEBS Lett. 265, 46–50.

    Article  PubMed  CAS  Google Scholar 

  26. Nunez, A. M., Berry, M., Imler, J. L., and Chambon, P. (1989) The 5′ flanking regions of the pS2 gene contains a complex enhancer region responsive to oestrogens, epidermal growth factor, a tumour promotor (TPA), the c-Has-ras oncoprotein and the c-jun protein.EMBO J. 8, 823–829.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. RIA Laboratory, Oncology Institute of Bari, Bari, Italy

    M. Correale, I. Abbate, C. D. Dragone, T. Tedone & M. D. Musci

  2. Division of Medical Oncology, Oncology Institute of Bari, Bari, Italy

    A. Paradiso, A. M. Catino & M. De Lena

  3. Division of Senology, Oncology Institute of Bari, Bari, Italy

    F. Schittulli

  4. Division of Gynaecological Oncology, Oncology Institute of Bari, Bari, Italy

    G. Gargano

Authors
  1. M. Correale
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Abbate
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Paradiso
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Schittulli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. D. Dragone
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Tedone
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Gargano
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. M. Catino
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. D. Musci
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. De Lena
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Correale, M., Abbate, I., Paradiso, A. et al. pS2—A new cytosolic protein recognized by monoclonal antibodies as a marker of hormone sensitivity in breast cancer. Cell Biophysics 22, 101–110 (1993). https://doi.org/10.1007/BF03033869

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03033869

Index Entries

Search

Navigation