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Analysis of gene expression upon DNA damage inArabidopsis

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Abstract

To understand their responses to DNA damage,Arabidopsis plants were exposed to methyl methanesulfonate (MMS). Such treatment inhibited growth and decreased the leaf chlorophyll content. A concomitant change in the expression of cell cyclerelated genes also occurred.CYCB1 expression was slightly increased but that ofCYCD1 declined. To identify the genetic elements in these responses, genome-wide transcription profiling ofArabidopsis was performed following the MMS treatment, using a cDNA microarray. Expression was altered by more than two-fold for 3666 genes — i.e., 1657 genes showed an increase and 2009 genes had a decrease in transcripts. Five DNA repair-related genes were slightly enhanced, while several disease resistance-related and glutathione transferase genes were strongly up-regulated. Interestingly, 27 ubiquitin-related genes were also altered by more than two-fold, suggesting that protein degradation may have been involved in those damage responses. These microarray results were validated by RT-PCR.

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Authors and Affiliations

  1. Molecular Signaling Laboratory, Department of Chemistry, Seoul National University, 151-742, Seoul, Korea

    Youn Sung Kim

  2. Laboratory of Plant Molecular Biology, Rockefeller University, 0021-3699, NY, USA

    Youn Sung Kim

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  1. Youn Sung Kim
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Correspondence to Youn Sung Kim.

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Kim, Y.S. Analysis of gene expression upon DNA damage inArabidopsis . J. Plant Biol. 49, 298–302 (2006). https://doi.org/10.1007/BF03031159

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  • DOI: https://doi.org/10.1007/BF03031159

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