Skip to main content
SpringerLink
Log in

Differential expression of two SOD (Superoxide dismytase) genes from small radish (Rhaphanus sativus L. var.sativus)

  • Published:
Journal of Plant Biology Aims and scope Submit manuscript

Abstract

We have isolated two Superoxide dismutase cDNA clones (RsCu/ZnSod andRsFeSod) from small radish (Raphanus sativus L.) by cDNA library screening.RsCu/ZnSod is 563 bp long, with an open reading frame of 153 amino acids, and corresponds to a protein of predicted molecular mass 15.1 kDa and a pl of 5.44. The 823-bp RsFeSod has an ORF of 213 amino acids, corresponding to a protein of predicted molecular mass 25.4 kDa and a pl of 8.77. Their nucleotide and deduced amino acid sequences show the highest homology with those ofArabidopsis. Genomic Southern blot analysis, using each cDNA clone as probe, has revealed that the SOD genes are present as at least two copies in the small radish genome. Nondenaturing polyacrylamide gels for SOD activity has demonstrated the presence of several isozymes, depending on the organ type and developmental stage. TheseRsSod genes also have differential expression patterns in response to treatments with white light, xenobiotics, UV, osmoticums, plant hormones, and salicylic acid. Therefore, we suggest that they are involved in an antioxidative defense mechanism against stress induced by environmental change.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  • Acevedo A, Scandalios JG (1991) Catalase and Superoxide dismutase gene expression and distribution during stem development in maize. Dev Genet12: 423–430

    Article  CAS  Google Scholar 

  • Alscher RG, Erturk N, Heath LS (2002) Role of Superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot53: 1331–1341

    Article  PubMed  CAS  Google Scholar 

  • Ausubel FM, Brent R, Kingston RE, Moore DD, Scidman JG, Smith JA, Struhl K (1987) Current Protocols in Molecular Biology. John Wiley and Sons, New York, pp 4.5.1–4.5.3

    Google Scholar 

  • Azevedo H, Lino-Nete T, Tavares RM (2004) Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait). Ann For Sci61: 847–850

    Article  CAS  Google Scholar 

  • Bannister JV, Bannister WH, Rotilio G (1987) Aspects of the structure, function and applications of Superoxide dismutase. CRC Crit Rev Biochem22: 111–180

    Article  PubMed  CAS  Google Scholar 

  • Baum JA, Scandalios JG (1981) Isolation and characterization of the cytosolic and mitochondrial Superoxide dismutase of maize. Arch Biochem Biophys206: 249–264

    Article  PubMed  CAS  Google Scholar 

  • Beauchamp CO, Fridovich I (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem44: 276–287

    Article  PubMed  CAS  Google Scholar 

  • Bowler C, Aliotte T, de Loose M, van Montagu M, Inze D (1989) The induction of manganese Superoxide dismutase in response to stress inNicotiana plumbaginifolia. EMBO J8: 31–38

    PubMed  CAS  Google Scholar 

  • Bowler C, van Camp W, van Montagu M, Inzé D (1994) Superoxide dismutase in plants. Crit Rev Plant Sci13: 199–218

    Article  CAS  Google Scholar 

  • Bowler C, van Montagu M, Inzé D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol43: 83–116

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem72: 248–254

    Article  PubMed  CAS  Google Scholar 

  • Cannon RE, Scandalios JG (1989) Two cDNAs encode two nearly identical Cu/Zn Superoxide dismutase proteins in maize. Mol Gen Genet219: 1–8

    Article  PubMed  CAS  Google Scholar 

  • Casano LM, Martin M, Sabater B (1994) Sensitivity of Superoxide dismutase transcript levels and activities to oxidative stress is lower in mature-senescent than in young barley leaves. Plant Physiol106: 1033–1039

    PubMed  CAS  Google Scholar 

  • Crowell DN, Amasino RM (1991a) Induction of specific mRNAs in, cultured soybean cells during cytokinin and auxin starvation. Plant Physiol95: 711–715

    Article  PubMed  CAS  Google Scholar 

  • Crowell DN, Amasino RM (1991b) Nucleotide sequence of an iron Superoxide dismutase complementary DNA from soybean. Plant Physiol96: 1393–1394

    Article  PubMed  CAS  Google Scholar 

  • Guan L, Scandalios JG (1998) Two structurally similar maize cytosolic Superoxide dismutase genes,Sod4 andSod4A, respond differentially to abscisic acid and high osmoticum. Plant Physiol117: 217–224

    Article  PubMed  CAS  Google Scholar 

  • Kaminaka H, Morita S, Tokumoto M, Yokoyama H, Masumura T, Tanaka K (1999) Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.). Biosci Biotechnol Biochem63: 302–308

    Article  PubMed  CAS  Google Scholar 

  • Kaminaka H, Morita S, Yokoi H, Masumura T, Tanaka K (1997) Molecular cloning and characterization of a cDNA for plastidic copper/zinc-superoxide dismutase in rice (Oryza sativa L.). Plant Cell Physiol38: 65–69

    PubMed  CAS  Google Scholar 

  • Kanematsu S, Asada K (1994) Superoxide dismutase,In F Toshio, S Kenji, eds, Molecular Aspects of Enzyme Catalysis. VCH, New York, pp 191–211

    Chapter  Google Scholar 

  • Kliebenstein DJ, Dietrich RA, Martin AC, Last RL, Dangl JL (1999) LSD1 regulates salicylic acid induction of copper zinc superoxide dismutase inArabidopsis thaliana. Mol Plant Microbe Inter-act12: 1022–1026

    Article  CAS  Google Scholar 

  • Kliebenstein DJ, Monde RA, Last RL (1998) Superoxide dismutase inArabidopsis: An eclectic enzyme family with disparate regulation and protein localization. Plant Physiol118: 637–650

    Article  PubMed  CAS  Google Scholar 

  • Kurepa J, Herouart D, van Montagu M, Inze D (1997) Differential expression of CuZn-and Fe Superoxide dismutase genes of tobacco during development, oxidative stress, and hormonal treatments. Plant Cell Physiol38: 463–470

    PubMed  CAS  Google Scholar 

  • Kwon SI, An CS (1999) Isolation and characterization of mitochondrial manganese Superoxide dismutase (MnSOD) fromCapsicum annuum L. Mol Cells9: 625–630

    PubMed  CAS  Google Scholar 

  • Kwon SI, An CS (2003) Cloning and expression of mitochondrial MnSOD from the small radish (Raphanus sativus L.). Mol Cells16: 194–200

    PubMed  CAS  Google Scholar 

  • Landry LG, Chappie CCS, Last RL (1995)Arabidopsis mutants lacking phenolic sunscreens exhibit enhanced ultraviolet-B injury and oxidative damage. Plant Physiol109: 1159–1166

    Article  PubMed  CAS  Google Scholar 

  • Lee HS, Kim KY, You SH, Kwon SY, Kwak SS (1999) Molecular characterization and expression of a cDNA encoding copper/zinc Superoxide dismutase from cultured cells of cassava (Manihot esculenta Crantz). Mol Gen Genet262: 807–814

    PubMed  CAS  Google Scholar 

  • Ma L, Li J, Qu L, Hager J, Chen Z, Zhao H, Deng XW (2001) Light control ofArabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell13: 2589–2607

    Article  PubMed  CAS  Google Scholar 

  • Mylona PV, Polidoros AN, Scandalios JG (1998) Modulation of antioxidant responses by arsenic in maize. Free Radic Biol Med25: 576–585

    Article  PubMed  CAS  Google Scholar 

  • Perl-Treves R, Galun E (1991) The tomato Cu/Zn Superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol Biol17: 745–760

    Article  PubMed  CAS  Google Scholar 

  • Perl-Treves R, Nacmias B, Aviv D, Zeelon ER Galun E (1988) Isolation of two cDNA clones from tomato containing two different Superoxide dismutase sequences. Plant Mol Biol11: 609–623

    Article  CAS  Google Scholar 

  • Sakaguchi S, Fukuda T, Takano H, Ono K, Takio S (2004) Photosynthetic electron transport differentially regulates the expression of Superoxide dismutase genes in liverwort,Marchantia paleacea var.diptera. Plant Cell Physiol39: 235–240

    Google Scholar 

  • Sakamoto A, Nosaka Y, Tanaka K (1993) Cloning and sequence analysis of a complementary DNA for manganese Superoxide dismutase from rice (Oryza sativa L.). Plant Physiol103: 1477–1478

    Article  PubMed  CAS  Google Scholar 

  • Sakamoto A, Ohsuga H, Tanaka K (1992) Nucleotide sequences of two cDNA clones encoding different Cu/Zn-superoxide dismutase from expressed in developing rice seed. Plant Mol Biol19: 323–327

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Ed 2, Cold Spring Harbor Laboratory Press, New York, pp 7–10

    Google Scholar 

  • Scandalios JG (1997) Molecular genetics of Superoxide dismutases in plants,In JG Scandalios, ed, Oxidative Stress and The Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, New York, pp 527–568

    Google Scholar 

  • Scandalios JG (2005) Oxidative stress: Molecular perception and transduction of signals triggering antioxidant gene defenses. Braz J Med Biol Res38: 995–1014

    Article  PubMed  CAS  Google Scholar 

  • Shin SY, Lee HS, Kwon SY, Kwon ST, Kwak SS (2005) Molecular characterization of a cDNA encoding copper/zinc Superoxide dismutase from cultured cells ofManihot esculenta. Plant Physiol Biochem43: 55–60

    Article  PubMed  CAS  Google Scholar 

  • Tanaka K, Takio S, Yamamoto I, Satoh T (1998) Characterization of a cDNA encoding Cu/Zn Superoxide dismutase from the liverwortMarchantia var.diptera. Plant Cell Physiol39: 235–240

    PubMed  CAS  Google Scholar 

  • Tsang EWT, Bowler C, Herouart D, van Camp W, Villaroel R, Genetello C, van Montagu M, Inze D (1991) Differential regulation of Superoxide dismutase in plants exposed to environmental stress. Plant Cell3: 783–792

    Article  PubMed  CAS  Google Scholar 

  • van Breusegem F, Slooten L, Stassart JM, Moens T, Botterman J, van Montagu M, Inze D (1999) Overproduction ofArabidopsis thaliana FeSOD confers oxidative stress tolerance to transgenic maize. Plant Cell Physiol40: 515–523

    PubMed  Google Scholar 

  • van Camp W, Bowler C, Villarroel R, Tsang EWT, Montagu MV, Inze D (1990) Characterization of iron Superoxide dismutase cDNAs from plants obtained by genetic complementation inEscherichia coll. Proc Natl Acad Sci USA84: 9903–9907

    Article  Google Scholar 

  • van Camp W, Capiau K, van Montagu M, Inze D, Slooten L (1996) Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol112: 1703–1714

    Article  PubMed  Google Scholar 

  • Wang FZ, Wang QB, Kwon SY, Kwak SS, Su WA (2005) Enhanced drought tolerance of transgenic rice plants expressing a pea manganese Superoxide dismutase. J Plant Physiol162: 465–472

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Ying Y, Chen J, Wang X (2004) TransgenicArabidopsis overexpressing Mn-SOD enhanced salt-tolerance. Plant Sci167: 671–677

    Article  CAS  Google Scholar 

  • White JA, Scandalios G (1988) Isolation and characterization of a cDNA for mitochondrial manganese Superoxide dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutase. Biochim Biophys Acta951: 61–70

    PubMed  CAS  Google Scholar 

  • Willekens H, van Camp W, van Montagu M, Inze D, Sandemann H, Langebartels C (1994) Ozone, sulfur dioxide, and Ultraviolet B have similar effects on mRNA accumulation of antioxidant genes inNicotiana plumbaginifolia L. Plant Physiol106: 1007–1014

    PubMed  CAS  Google Scholar 

  • Williamson JD, Scandalios JG (1992) Differential response of maize catalases and Superoxide dismutase to the photoactivated fungal toxin cercosporin. Plant J2: 351–358

    PubMed  CAS  Google Scholar 

  • Zhu D, Scandalios JG (1994) Differential accumulation of manganese-superoxide dismutase transcripts in maize in response to abscisic acid and high osmoticum. Plant Physiol106: 173–178

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Seoul National University, 151-747, Seoul, Korea

    Soon Il Kwon & Chung Sun An

Authors
  1. Soon Il Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chung Sun An
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soon Il Kwon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kwon, S.I., An, C.S. Differential expression of two SOD (Superoxide dismytase) genes from small radish (Rhaphanus sativus L. var.sativus). J. Plant Biol. 49, 477–483 (2006). https://doi.org/10.1007/BF03031129

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03031129

Keywords

Search

Navigation