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Low expression profiles of heat stress-related genes inCapsicum annuum

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Abstract

A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5′ ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about 41% of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs (15%) were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising 1% of the ESTs. Genes related to energy and protein fates constituted the second (10%) and third (9%) largest groups, respectively. Finally, 3% of the ESTs were assigned to transcription, and 2% to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.

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Authors and Affiliations

  1. Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, 151-742, Seoul, Korea

    M. Ashrafuzzaman & Choo Bong Hong

  2. Department of Pharmacology, Pharmacogenomics Research Center, College of Medicine, Inje University, 614-735, Busan, Korea

    S. June Oh

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  1. M. Ashrafuzzaman
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  2. S. June Oh
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Correspondence to Choo Bong Hong.

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Ashrafuzzaman, M., Oh, S.J. & Hong, C.B. Low expression profiles of heat stress-related genes inCapsicum annuum . J. Plant Biol. 48, 85–95 (2005). https://doi.org/10.1007/BF03030567

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