Abstract
The combination of specific enzymic degradation with GC/MS or LC/MS identification and quantitation of enantiomeric diacylglycerols and reverse isomers has greatly improved the methods of structural analysis of triacylglycerols, so that in many instances complete characterization of both major and minor species is possible. The techniques described for the analysis of triacylglycerols and sn-1,2- and sn-2,3-diacylglycerols are also applicable to the X-1,3-diacylglycerols and X-1-monoacylglycerols following coversion to triacylglycerols by acylation with appropriate fatty acids. For many applications, however, a combination of specific enzymic hydrolysis with a GLC analysis of the products on polar capillary columns may be adequate for the identification and quantitation of the major molecular species of both triacylglycerols and diacylglycerols.
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References
Kuksis, A., J.J. Myher and L. Marai. JAOCS 61:1582 (1984).
Brockerhoff, H.J. Lipid Res. 1:10 (1965).
Christie, W.W. Lipid Analysis, Pergamon Press, Oxford, Great Britain. (1973).
Myher, J.J., and A. Kuksis. Can. J. Biochem. 57:117 (1979).
Kuksis, A., in Lipid Chromatographic Analysis (Marinetti, G.V., ed.), Marcel Dekker, New York, NY. (1967) pp. 239–337.
Myher, J.J., A. Kuksis, W.C. Breckenridge and J.A. Little. Lipids 19:683 (1984).
Myher, J.J., A. Kuksis and G. Steiner. Ibid. 19:673 (1984).
Myher, J.J., A. Kuksis, S.C. Vasdev and K.J. Kako. Can. J. Biochem. 57:1315 (1979).
Kuksis, A., L. Marai and D.A. Gornall. J. Lipid Res. 8:352 (1967).
Perkins, E.G., and P.V. Johnston, Lipids 4:301 (1969).
Myher, J.J., and A. Kuksis. Can. J. Biochem. 60:638 (1982).
Myher, J.J., and A. Kuksis. Can. J. Biochem. Cell Biol. 62:352 (1984).
Yurkowski, M., and H. Brockerhoff. Biochim. Biophys. Acta 125:55 (1966).
Manganaro, F., J.J. Myher, A. Kuksis and D. Kritchevsky. Lipids 16:508 (1981).
Myher, J.J., A. Kuksis, W.C. Breckenridge, V. McGuire and J.A. Little. Ibid. 20:90 (1985).
Pind, S., A. Kuksis and J.J. Myher. (1983) (Unpublished results).
Bugaut, M., J.J. Myher, A. Kuksis and A.G.D. Hoffman. Biochim. Biophys. Acta 792:254 (1984).
Kuksis, A., L. Marai and J.J. Myher. J. Chromatogr. Biomed. Applic. 273:43 (1983).
Brockerhoff, H. Lipids 6:942–956 (1971).
Myher, J.J., L. Marai, A. Kuksis and D. Kritchevsky. Ibid. 12:775 (1977).
Morley, N.H., and A. Kuksis. J. Biol. Chem. 247:6389 (1972).
Paltauf, F., F. Esfandi and A. Holasek. FEBS Letters 40:119 (1974).
Snyder, F., in Ether Lipids (Snyder, F., ed.) Academic Press, New York, NY, (1972) pp. 273–295.
Warner, H.R., and W.E.M. Lands. JAOCS 85:60 (1963).
Polheim, D., J.S.K. David, M. Schultz, M.B. Wylie and J.M. Johnston. J. Lipid Res. 14:415 (1973).
Bugaut, M., A. Kuksis and J.J. Myher. (1984) Unpublished results.
Paltauf, F., and E. Wagner. Biochim. Biophys. Acta 431:359 (1976).
Litchfield, C. Analysis of Triglycerides, Academic Press, New York, NY (1972).
Kuksis, A. Progress Chem. Phys. Fats Other Lipids 12:1 (1972).
Holub, B.J., and A. Kuksis. Adv. Lipid Res. 16:1 (1978).
Mills, S.C., L.J. Cook, T.W. Scott and P.J. Nestel. Lipids 11:49 (1975).
Bezard, J.A., and M.A. Ouedraogo. J. Chromatogr. 196:279 (1980).
Manganaro, F., J.J. Myher and A. Kuksis. JAOCS 60:734, Abs. No. 225 (1983).
Kuksis, A., J.J. Myher and L. Marai. JAOCS 62:767 (1985).
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Kuksis, A., Myher, J.J. & Marai, L. Lipid methodology — chromatography and beyond. Part II. GC/MS, LC/MS and specific enzymic hydrolysis of glycerolipids. J Am Oil Chem Soc 62, 762–767 (1985). https://doi.org/10.1007/BF03028750
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DOI: https://doi.org/10.1007/BF03028750