Skip to main content
SpringerLink
Log in

Comparative studies of triacylglycerol structure of very low density lipoproteins and chylomicrons of normolipemic subjects and patients with type II hyperlipoproteinemia

  • Published:
Lipids

Abstract

The triacylglycerols of very low density lipoproteins (VLDL) and of chylomicrons were analyzed in the fasting and postabsorptive states from normolipemic subjects and patients with Frederickson's Type II hyperlipoproteinemia, who subsisted on free choice diets, standard diets excluding lard, or were given a breakfast enriched in lard. The VLDL and chylomicrons were obtained by conventional ultracentrifugation, and the triacylglycerols were isolated by thin-layer chromatography (TLC). Representative sn-1,2, 2n-2-3- and sn-1,3-diacylglycerols were generated by partial Grignard degradation of the triacylglycerols and a stereospecific hydrolysis by phospholipase C of the mixed sn-1,2(2,3)-diacyl phosphatidylcholines prepared as intermediates. Representative sn-2-acylglycerols were obtained by hydrolysis with pancreatic lipase. Positional distribution of the fatty acids was established by subtracting in turn the fatty acid composition of the sn-2-position from the fatty acid composition of the sn-1,2- and sn-2,3-diacylglycerols. The molecular association of the fatty acids in the diacylglycerol moieties was determined by gas-liquid chromatography with mass spectrometry (GC/MS) of the tertiary-butyldimethylsilyl (t-BDMS) ethers. The molecular association of the fatty acids in the triacylglycerols was determined by 1-random 2-random 3-random calculation following experimental validation of the distribution. The results confirm a marked asymmetry in the positional distribution of the fatty acids in all triacylglycerol samples, with the palmitic acid predominantly in the sn-1-position, the unsaturated acids about equally divided between the sn-2-and sn-3-positions, and the stearic acid divided about equally between the sn-1- and sn-3-positions. The overall structure of the VLDL and chylomicron triacylglycerols from patients and control subjects was characterized by a noncorrelative distribution of fatty acids under all dietary conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allard, C., Davignon, J. Marcel, Y.L., Goulet, C., Kuba, K., Alteresco, M., Caradec, Y., and Plante, D. (1973) Can. J. Biochem. 51, 1509–1514.

    PubMed  CAS  Google Scholar 

  2. Parijs, J., De Weerdt, G.A., Beke R., and Barbier, F. (1974) Lipids 9, 937–938.

    Article  PubMed  CAS  Google Scholar 

  3. Gordon, D.T., Pitas, R.E., and Jensen, R.G. (1975) Lipids 10, 270–283.

    Article  PubMed  CAS  Google Scholar 

  4. Parijs, J., De Weerdt, G.A., Beke, R., and Barbier, F. (1976) Clin. Chim. Acta 66, 43–52.

    Article  PubMed  CAS  Google Scholar 

  5. Jensen, R.G., Clark, R.M., Gerrior, S.A., Fey, M.B., and Gotto, A.M., Jr. (1979) Lipids 14, 691–694.

    PubMed  CAS  Google Scholar 

  6. Myher, J.J., Kuksis, A., Breckenridge, W.C., and Little, J.A. (1984) Lipids 19, 683–691.

    PubMed  CAS  Google Scholar 

  7. Myher, J.J., Kuksis, A., Breckenridge, W.C., and Little, J.A. (1981), in Abstract ISF/AOCS World Congress, New York, April 27–May 1, 1981. Abstract No. 413.

  8. Myher, J.J., Marai, L., Kuksis, A., and Kritchevsky, D. (1977) Lipids 12, 775–785.

    Article  PubMed  CAS  Google Scholar 

  9. Lipid Research Clinics Program. Manual of Laboratory Operations. Vol. 1: Lipid and Lipoprotein Analysis. DHEW Publication Number (NIH) 75-628. Washington, DC, National Institues of Health, 1974.

    Google Scholar 

  10. Hatch, F.T., and Lees, R.S. (1968) Adv. Lipid Res. 6, 1–68.

    PubMed  CAS  Google Scholar 

  11. Kuksis, A., Myher, J.J., Geher, K., Hoffman, A.G.D., Breckenridge, W.C., Jones, G.J.L., and Little, J.A. (1978) J. Chromatogr. Biomed. Applic. 146, 393–412.

    Article  CAS  Google Scholar 

  12. Lowry, O.H., Rosebrough, N.J., Farr, A.K., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  13. Myher, J.J. and Kuksis, A. (1979) Can. J. Biochem. 57, 117–124.

    PubMed  CAS  Google Scholar 

  14. Yurkowski, M., and Brockerhoff, H. (1966) Biochim. Biophys. Acta 125, 55–59.

    PubMed  CAS  Google Scholar 

  15. Thomas, A.E., III, Scharoun, J.E., and Ralston, H. (1965) J. Am. Oil Chem. Soc. 42, 789–792.

    CAS  Google Scholar 

  16. Baer, E., and Kindler, A. (1962) Biochemistry 1, 518–520.

    Article  PubMed  CAS  Google Scholar 

  17. Skipski, V.P. Peterson, R.F., and Barclay, M. (1964) Biochem. J. 90, 374–378.

    PubMed  CAS  Google Scholar 

  18. Mattson, F.H., and Volpenhein, R. (1961) J. Lipid Res. 3, 58–62.

    Google Scholar 

  19. Kuksis, A., Marai, L., and Myher, J.J. (1973) J. Am. Oil Chem. Soc. 50, 193–201.

    PubMed  CAS  Google Scholar 

  20. Myher, J.J., Kuksis, A., Marai, L., and Yeung, S.K.F. (1978) Can. J. Biochem. 50, 557–561.

    CAS  Google Scholar 

  21. Kuksis, A., Breckenridge, W.C., Myher, J.J., and Kakis, A. (1978) Can. J. Biochem. 56, 630–639.

    Article  PubMed  CAS  Google Scholar 

  22. Myher, J.J., Marai, L., and Kuksis, A. (1974) Anal. Biochem. 62, 188–203.

    Article  PubMed  CAS  Google Scholar 

  23. Bugaut, M., Myher, J.J., Kuksis, A., and Hoffman, A.G.D. (1984) Biochem. Biophys. Acta 792, 254–269.

    PubMed  CAS  Google Scholar 

  24. Manganaro, F., Myher, J.J., and Kuksis, A. (1983) J. Am. Oil Chem. Soc. 60, 735. Abs. No. 225.

    Google Scholar 

  25. Possmayer, F., Scherphof, G.L., Dubbelman, T.N.A.R., and Van Deenen, L.L.M. (1969) Biochim. Biophys. Acta 176, 95–110.

    PubMed  CAS  Google Scholar 

  26. Yamashita, S., Hosaka, K., and Noma, S. (1973) Eur. J. Biochem. 38, 25–31.

    Article  PubMed  CAS  Google Scholar 

  27. Lands, W.E.M., Pieringer, R.A., Slakey, P.M., and Zschocke, A. (1966) Lipids 1, 444–448.

    Article  CAS  PubMed  Google Scholar 

  28. Slakey, P.M., and Lands, W.E.M. (1968) Lipids 3, 30–36.

    Article  CAS  PubMed  Google Scholar 

  29. Wood, R., and Harlow, R.D. (1969) Arch. Biochem. Biophys. 131, 495–501.

    Article  PubMed  CAS  Google Scholar 

  30. Wood, R., and Snyder, F. (1969) Arch Biochem. Biophys. 131, 478–494.

    Article  PubMed  CAS  Google Scholar 

  31. Christie, W.W., and Moore, J.H. (1970) Biochim. Biophys. Acta 210, 46–56.

    PubMed  CAS  Google Scholar 

  32. Kane, J.P., Hardman, D.A., and Paulus, H.E. (1980) Proc. Natl. Acad. Sci., U.S.A. 77, 2465–2469.

    Article  PubMed  CAS  Google Scholar 

  33. Morley, N.H., and Kuksis, A. (1972) J. Biol. Chem. 247, 6389–6393.

    PubMed  CAS  Google Scholar 

  34. Paltauf, F., Esfandi, F., and Holasek, A. (1974) FEBS Letters 40, 119–123.

    Article  PubMed  CAS  Google Scholar 

  35. Akesson, B., Gronowitz, S., and Herslof, B. (1976) FEBS Letters 71, 241–244.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada

    J. J. Myher, A. Kuksis, W. C. Breckenridge, V. McGuire & J. A. Little

  2. Department of Medicine, University of Toronto, Toronto, Ontario, Canada

    J. J. Myher, A. Kuksis, W. C. Breckenridge, V. McGuire & J. A. Little

Authors
  1. J. J. Myher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Kuksis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. C. Breckenridge
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. McGuire
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. A. Little
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Myher, J.J., Kuksis, A., Breckenridge, W.C. et al. Comparative studies of triacylglycerol structure of very low density lipoproteins and chylomicrons of normolipemic subjects and patients with type II hyperlipoproteinemia. Lipids 20, 90–101 (1985). https://doi.org/10.1007/BF02534214

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02534214

Keywords

Search

Navigation