Abstract
Adriamycin resistant cells were obtained from low dosage treated BABL/c mice inoculated with S-180 cells. Resistance of these cells for adriamycin was 66-fold more than their parental cells. The resistance for a typical DNA topoisomerase II inhibitor VP16 (Etoposide) was increased 9 times. Overexpression of multidrug resistant gene (MDR gene) products, P-glycoproteins (P-l 70), was also demonstrated by immunohistochemistry. Furthermore, the ability of the resistant cells to reduce net cellular drug accumulation measured by flow fluorescence cytometry was 89-fold higher than their parental cells. These results support the hypothesis that the resistance of S-180R cells to adriamycin was mainly due to the overexpression of P-glycoproteins. The S- 180R cells will be useful to select drugs or some other therapeutic strategies to overcome multidrug resistancein vivo.
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Zhang, T., Gao, C., Zhang, L. et al. Development of an adriamycin resistant murine tumor S-180 cell lineIn vivo . Chin J Cancer Res 5, 240–244 (1993). https://doi.org/10.1007/BF03023753
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DOI: https://doi.org/10.1007/BF03023753