Abstract
Purpose: The mechanism responsible for the mediation of hypertension in response to increased desflurane levels is unclear. This study compared the effect of desflurane and halothane on phenylephrine (PE)-induced contraction in rat aorta ring and the effect of desflurane in the presence and absence of nitric oxide (NO) synthase activity.
Methods: Endothelium-free rat aorta rings were exposed serially to 10−7M, 10−6M and 10−5M PE alone and subsequently in the presence of 2 MAC desflurane and halothane. Secondly, endothelium-free preparations were exposed to 10−6M PE serially in the presence of 0, 1, 2 and 3 MAC desflurane and halothane. Thirdly, using an endothelium-intact preparation, the effect of desflurane on PE-induced contraction was examined, in the presence or absence of NG-nitrol-L-argine (L-NNA), an inhibitor of constitutive and inducible NO synthase.
Results: Contraction amplitudes secondary to 10−6 and 10−5M PE in endothelium-free preparations were increased by 74% and 36% respectively (P<0.05) in the presence of 2 MAC desflurane compared to controls. In endothelium- free preparations, contraction amplitudes secondary to 10−6M PE were increased in the presence of 1 and 2 MAC desflurane by 32% and 18% respectively (P <0.05) and reduced by 16% in the presence of 3 MAC halothane (P <0.05). In endothelium-intact preparations an expected absolute increase in contraction amplitude occurred in the presence of L-NNA but the desflurane effect was detectable both in the presence and absence of L-NNA.
Conclusion: Our results suggest that desflurane may have a local vasoconstrictive effect independent of endothelium and NO synthase activity. The mechanism remains to be determined.
Résumé
Objectif: Le mécanisme responsable de la médiation de l’hypertension en réponse à l’augmentation de desflurane n’est pas encore connu. La présente étude a comparé l’effet du desflurane et de l’halothane sur les contractions induites par la phényléphrine (PE). dans des anneaux d’aorte de rat, et l’effet du desflurane avec et sans actimité de la synthétase de l’oxyde nitrique (NO).
Méthode: Des anneaux d’aorte de rat sans exdothélium ont été exposés par série à de la PE de 10−7M, 10−6M et 10−5M et, par la suite, à du desflurane et à de l’halothane à 2 CAM. Les préparations sans endothélium ont été ensuite exposées par série à de la PE a 10−6M en présence de desflurane et d’halothane à 0, 1, 2 et 3 CAM, Enfin, en utilisant une préparation dont l’endothélium a été conservé intact, l’effet du desflurane sur la contraction induite par la PE a été examiné, en présence et en l’absence de NG-nitro-L-arginine (L-NNA), un inhibiteur de la synthétase de NO constitutive et inductible.
Résultats: L’amplitude des contractions secondaires à la présence de PE à 10−6 et à 10−5 dans des préparations sans endothélium a augmenté de 74 % et 36 %, respectivement (P<0,05) avec du desflurane à 2 CAM, en comparalson avec les témoins. Dans les préparations sans endothélium, l’amplitude des contractions liées à de la PE à 10−6 s’est élevée en présence de desflurane à 1 et 2 CAM, de 32 % et de 18 %, respectivement (P<0,05) et s’est abaissée de 16 % en présence d’halothane à 3 CAMP (P<0,05). Dans les préparations à l’endothélium intact, une augmentation absolute présumée de l’amplitude des contractions est survenue en présence de L-NNA, mais l’effet du desflurane a été détectable en présence et en l’absence de L-NNA.
Conclusion: Nos résultats suggèrent que le desflurane pourrait avoir un effet vasoconstricteur local indépendant de l’endothélium et de l’activité de la synthétase de NO. Le mécanisme reste à déterminer.
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Griffin, M.J., Breen, P.M., O’Connor, J.J. et al. Desflurane, compared to halothane, augments phenylephrine-induced contraction in isolated rat aorta smooth muscle. Can J Anesth 48, 361–368 (2001). https://doi.org/10.1007/BF03014964
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DOI: https://doi.org/10.1007/BF03014964