Abstract
Purpose
To examine the effect of halothane on the cytosolic Ca2+ concentration ([Ca2+]i)-tension relationship of rat aortic smooth muscle.
Methods
Rat aortic rings without endothelia were loaded with the fluorescent Ca2+ indicator, Fura PE3-AM, and then mounted in organ baths. The changes in isometric tension and [Ca2+]i were measured simultaneously. In one series ionomycin (10 nM–3 μM) was added to normal Krebs’ solution cumulatively in the absence and presence of halothane ( 1.5%, 3%). In the other series, CaCl2 (0.3–3 mM) was added to Ca2+-free Krebs’ solution including high KCl (50 mM), phenylephrine (100 nM) or prostaglandin F2α (PGF2α, 1–3 μM) in the absence and presence of halothane (1.5%, 3%). The linear part of [Ca2+]i-tension relationship was analyzed by a linear regression.
Results
Halothane, 1.5%, had no effect on the normal [Ca2+]i-tension relationship obtained with the calcium ionophore, ionomycin (10 nM–3 μM), but halothane 3% decreased the slope of the relationship (0.239 ± 0.037 for control and 0.110 ± 0.010 for halothane 3%,P < 0.05). Halothane, 1.5% and 3%, did not change the [Ca2+]i-tension relationship obtained with CaCl2 (0.3–3 mM) in the presence of high KCl (50 mM) or phenylephrine ( 100 nM). In contrast, halothane, 3%, inhibited the intercept of [Ca2+]i-tension relationship obtained with CaCl2 (0.3–3 mM) in the presence of prostaglandin F2α (PGF2α′, 1–3 μM) (45.708 ± 4.233 for control and 26.997 ± 2.522 for halothane 3%, P < 0.0l).
Conclusion
Halothane decreases the Ca2+ sensitivity and that in the presence of PGF2.
Résumé
Objectif
Étudier l’effet de l’halothane sur la relation tension isométrique-concentration calcique cytosolique ([Ca2+]i) sur le muscle lisse de l’aorte chez le rat.
Méthode
Des anneaux aortiques de rat, sans endothélium, ont été chargés avec un indicateur de fluorescence pour le Ca2+, le Fura PE3-AM, et montés ensuite dans des bains d’organes. Les changements de tension isométrique et de [Ca2+]i ont été mesurés simultanément. Dans une série, on a ajouté de l’ionomycine (10 nM–3 μM) à la solution Krebs normale, de façon cumulée en l’absence et en présence d’halothane (1,5 %, 3 %). Dans l’autre série, du CaCl2 (0,3–3 mM) a été ajouté à la solution Krebs libre de Ca2+ incluant une forte concentration de KCl (50 mM), de phényléphrine (100 nM) ou de prostaglandine F2 (PGF2, 1–3 μM) en l’absence et en présence d’halothane (1,5 %, 3 %). La portion linéaire de la relation [Ca2+]i-tension a été analysée par une régression linéaire.
Résultats
]L’halothane, 1,5%, n’a pas eu d’effet sur la relation [Ca2+]i-tension isométrique normale obtenue avec l’ionophore calcique (ionomycine 10 nM–3 μM), mais l’halothane 3% a fait baisser la pente de la relation (0,239 ± 0,037 pour les témoins et 0, 110 ± 0,010 pour l’halothane 3%, P < 0,05). Lhalothane, 1,5% et 3%, n’a pas changé la relation [Ca2+]i-tension obtenue avec le CaCl2 (0,3–3 mM) en présence d’une forte concentration de KCI (50 mM) ou de phényléphrine (100 nM). Par ailleurs, l’halothane 3% modifie à la baisse l’ordonnée à l’origine de la relation [Ca2+]i-tension obtenue avec le CaCI2 (0,3-3 mM) en présence de prostaglandine F2 (PGF2, 1–3 μM) (45,708 ± 4,233 pour les témoins et 26,997 ± 2,522 pour l’halothane 3 %,P < 0,01).
Conclusion
L’halothane diminue la sensibilité du Ca2+ et ce, en présence de la PGF2.
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Kakuyama, M., Nakamura, K. & Mori, K. Halothane decreases calcium sensitivity of rat aortic smooth muscle. Can J Anesth 46, 1164–1171 (1999). https://doi.org/10.1007/BF03015527
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DOI: https://doi.org/10.1007/BF03015527