Summary
Cyclopropane, halothane, and methoxyflurane were investigated for their effects on the viscometry of freshly drawn human blood (group O, Rh+). A total of 43 blood and 63 plasma samples containing specific anaesthetic agents were studied under controlled conditions of temperature, pH, Pco2, Po2, and red blood cell and plasma protein concentrations. A series of three cone plate viscometers, each with different shear rate ranges from 1500 to 6.75 sec.-1, was used. The Casson viscosity of blood and apparent plasma viscosity were determined from the slope of the shear stress-shear rate relationship plotted on a square root scale. Yield stress was determined by extrapolation from the shear stress axis intercept. Our findings indicate that the three anaesthetic agents, within concentrations equivalent to those used during clinical anaesthesia, caused no discernible effect on either yield stress or the apparent viscosity of blood or plasma. However, there was a marginal increase of the yield stress of blood with high concentrations of cyclopropane (above 20 mg.%) and a trend toward reduced plasma viscosity in all of the methoxyflurane samples.
Résumé
Nous avons étudié les effets du cyclopropane, de l’halothane et du méthoxyflurane sur la viscométrie du sang humain fraichement prélevé (groupe O, Rh positif). Nous avons fait nos études sur quarante-trois échantillons de sang et soixante-trois échantillons de plasma contenant spécifiquement ces agents anes-thésiques; l’étude a été faite aux mêmes conditions de température, de pH, de Pco2 et de concentration en protéines des globules rouges et du plasma. Nous avons fait des séries de trois viscomètres cone-plate, chacun possédant différentes échelles ”shear rate” de 1500 — 6.75 sec.-1. Nous avons précisé la viscosité Casson du sang et la viscosité apparente du plasma à partir de la courbe de relation ”shear stress-shear rate” placée sur une échelle de racine carrée. Le ”yield stress” a été obtenu par l’extrapolation à partir du croisement de Taxe du ”shear stress”. Nos résultats nous apprennent que, à des concentrations équivalentes à celles utilisées en anesthésie clinique, ces trois agents anesthésiques ne produisent aucun effet décelable ni sur le ”yield stress” ni sur la viscosité apparente du sang ou du plasma. Toutefois, avec une haute concentration sanguine de cyclopropane (au-dessus de 20 mg.%), nous avons observé une faible augmentation du ”yield stress” du sang; de plus, dans tous les échantillons avec le méthoxyflurane, nous avons observé une tendance à la diminution de la viscosité du plasma.
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This work was supported by grants HE 01711 and HE 08783 from the National Institutes of Health, U.S. Public Health Service.
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Aronson, H.BZ., Levesque, P.R., Charm, S. et al. Influence of anaesthetics on rheology of human blood. Can. Anaesth. Soc. J. 15, 244–257 (1968). https://doi.org/10.1007/BF03008737
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DOI: https://doi.org/10.1007/BF03008737