Abstract
A series of bioassays such as sister chromatid exchange frequencies (SCEs), chromosomal aberration (CA), micronuclei rate (MN) and cell-cycle delay have been used to detecting the genotoxic effect of cigarette smoke condensate (CSC) on human diploid cell 2BS strain. The results suggested that a higher SCE, (17.0/cell) was observed in 2BS cells treated with CSC at 100 µg/ml, as compared with 6.9/cell of the background (P<0.001). CA rate was significantly increased from 4% to 36% in cells treated with 10 µg/ml CSC (P<0.001). MN rate varied from 9–26‰. in cells treated with CSC compared to that of control (6‰). Meanwhile, the cell-cycle of cells was markedly delayed by CSC. The survival rate of 2BS cells declined to 59.6% for treatment with CSC at 200 µg/ ml. There was a dose-effect response in SCEs, CA, MN rate. We proposed that active oxygen might responsible for genotoxicity of CSC on cells.
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Gu, M., Hu, G. & Luo, X. Genotoxic effect of cigarette smoke condensate (CSC) on human diploid cell 2BS strain. Chin J Cancer Res 4, 39–42 (1992). https://doi.org/10.1007/BF02997508
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DOI: https://doi.org/10.1007/BF02997508