Abstract
A new microbial electrode using immobilizedClostridium butyricum was prepared for biochemical oxygen demand (BOD) estimation of wastewaters. The current of the electrode was decreased with time until a steady state was reached. The steady state current was in all cases attained within 30-40 min at 37°C, and the maximum current output was obtained at 37°C and pH between 6.2 and 7.0. A linear relationship was obtained between the steady state current and BOD. The steady state current values were reproducible within ±7% of the relative error. The BOD of industrial wastewaters can be estimated by using the microbial electrode. Relative error of the BOD estimation of industrial wastewaters was within ± 10%. The current output of the microbial electrode was almost constant for 30 days.
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Karube, I., Matsunaga, T. & Suzuki, S. A new microbial electrode for BOD estimation. Journal of Solid-Phase Biochemistry 2, 97–104 (1977). https://doi.org/10.1007/BF02991399
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DOI: https://doi.org/10.1007/BF02991399