Abstract
A technique for the rapid and accurate estimation of nonheme iron using inductively coupled plasma-atomic emission spectrometry is described. Yttrium was used as an internal standard. An external calibration method was used. The standards were prepared in a matrix composed of 2.5N HCl in 10% (w/v) trichloroacetic acid. The supernatant and coagulum fractions of liver nonheme iron were separated by the method of Drysdale and Ramsay with minor modification (13). The data determined by this procedure was compared and found to be agreement with data determined by the method of Hallgren (12). To evaluate the iron status of rats, hemoglobin and liver nonheme iron were determined. Hemoglobin and all of the nonheme iron fractions of the rats fed an iron-deficient diet were significantly lower than those of the rats fed an iron-sufficient diet. The blood content in the liver was estimated to be 80 μL/g from the blood iron concentration, and the difference between total and nonheme iron concentration in liver.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
H. N. Munro and M. C. Linder,Physiol. Rev. 58, 317–396 (1978).
B. W. Gabrio, A. Shoden, and C. A. Finch,J. Biol. Chem. 204, 815–821 (1953).
D. Drabkin,Physiol. Rev. 31, 345–431 (1951).
H. G. du Défaix, R. Puente, B. Vidal, E. Pérez, and H. Vidal,Am. J. Clin. Nutr. 33, 133–136 (1980).
S. C. Finch and C. A. Finch,Medicine 34, 381–430 (1955).
S. M. Ahmed, M. Kimura, and Y. Itokawa,Trace Nutrients Research (Kyoto, Japan) 4, 121–126 (1988).
M. Kimura and Y. Itokawa,Magnesium in Health and Disease, Y. Itokawa and J. Durlach, eds., John Libbey, London & Paris, pp. 95–102 (1989).
T. L. Sourkes, K. Lloyd, and H. Birnbaum,Can. J. Biochem. 46, 267–271. (1968).
F. H. Nielsen, S. H. Givand, and D. R. Myron,Fed. Proc. 34, 923 (1975).
K. Yokoi, M. Kimura, and Y. Itokawa,Biol. Trace Elem. Res. 24, 223–231 (1990).
J. W. Drysdale and H. N. Munro,Biochem. J. 95, 851–858 (1965).
B. Hallgren,Acta Soc. Med. 59, 79–208 (1954).
J. W. Drysdale and W. N. M. Ramsay,Biochem. J. 95, 282–288 (1965)
S. Greenfield,Analyst (London) 105, 1032–1044 (1980).
W. J. Boyko, P. N. Keliher, and J. M. Malloy,Anal. Chem. 52, 53R-69R (1980).
G. J. Schmidt and W. Slavin,Anal. Chem. 54, 2491–2495 (1982).
V. Laufberger,Bull. Soc. Chim. Biol. 19, 1575–1582 (1937).
J. A. Millar, R. L. C. Cumming, J. A. Smith, and A. Goldberg,Biochem. J. 119 643–649 (1970).
D. A. Lipschitz, T. H. Bothwell, H. C. Seftel, A. A. Wapnick, and R. W. Charlton,Brit. J. Haematol 20, 155–163 (1971).
C. P. Van Wyk, M. Linder-Horowitz, and H. N. Munro,J. Biol. Chem. 246, 1025–1031 (1971).
A. Vidnes and L. Holgeland,Biochem. Biophys. Acta 328, 365–372 (1973).
A. Shukla, K. N. Agarwal, and G. S. Shukla,Biol. Trace Elem. Res. 22, 141–152 (1989).
E. H. Morgan,J. Physiol. (London) 158, 573–586 (1961).
J. C. Wyllie and N. Kaufman,Brit. J. Haematol. 20, 321–327 (1971).
E. M. Scott and R. H. McCoy,Arch. Biochem. 5, 349–355 (1944).
A. I. Lau and M. L. Failla,J. Nutr. 114, 224–233 (1984).
A. R. Sherman, H. A. Guthrie, and I. Wolinsky,Proc. Soc. Exptl. Biol. Med. 156, 396–401 (1977).
M. Uehira, Y. Endo, G. Mowlah, K. Suzuki, and S. Goto,Nutr. Rep. Int. 35, 1023–1034 (1987).
A. Robertson, J. N. Morrison, A. M. Wood, and I. Bremner,J. Nutr. 119, 439–445 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yokoi, K., Kimura, M. & Itokawa, Y. Determination of nonheme iron using inductively coupled plasma-atomic emission spectrometry. Biol Trace Elem Res 31, 265–279 (1991). https://doi.org/10.1007/BF02990196
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02990196