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Transport of selenate and selenite across the brush border membrane of rat and sheep small intestine

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Abstract

Mucosal uptake of75Se-labeled selenate and selenite across the brush border was investigated in sheep and rat small intestine, using 3-min mucosal exposures. Uptake of selenate and selenite occurred faster in rat than in sheep small intestine. With the exception of sheep duodenum, mucosal selenate uptake was Na+-dependent in sheep and rat small intestine. Mucosal uptake of selenite across the brush border was Na+-dependent only in sheep midjejunum, whereas it was Na+-independent in sheep duodenum and ileum and the rat whole small intestine. Various anions inhibited selenate transport in the presence of Na+ in sheep midjejunum in the order S2O2 2- = CrO4 2- > MoO4 2- and in rat ileum in the order CrO4 2- = S2O3 2- > SC4 2- > MoO4 2-. Thiosulfate also inhibited mucosal selenite uptake in the presence of Na+ in sheep midjejunum. Preincubation of rat ileum with glutathione (GSH) enhanced mucosal selenite uptake, whereas selenate uptake remained unaffected.

These results indicate that selenate transport across the brush border membrane is energized in part by the Na+-gradient. Moreover, the Na+-dependent transport mechanism for the Se salts apparently has an affinity for other anions (S2O3 2-, SO4 2-, CrO4 2-, MOo4 2-). The findings further indicate that intracellular GSH plays a role in the absorption of selenite, probably by an increase of intracellular selenite metabolism. The Na+-independent mucosal uptake of selenate and selenite probably represents diffusion.

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Authors and Affiliations

  1. Institut für Veterinär-Physiologie der Universität Zürich, Winterthurerstrasse 260, 8057, Zürich, Switzerland

    F. Ardüser, S. Wolffram, E. Scharrer & B. Schneider

Authors
  1. F. Ardüser
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  2. S. Wolffram
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  3. E. Scharrer
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  4. B. Schneider
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Ardüser, F., Wolffram, S., Scharrer, E. et al. Transport of selenate and selenite across the brush border membrane of rat and sheep small intestine. Biol Trace Elem Res 9, 281–290 (1986). https://doi.org/10.1007/BF02988826

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  • DOI: https://doi.org/10.1007/BF02988826

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