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Uptake and metabolism of 2,4,6-trinitrotoluene in higher plants

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Abstract

The fate of the explosive 2,4,6-TNT in plants is of major interest. Therefore, a method was developed to analyse TNT and derivatives in plant tissue. The method was utilized to investigate the uptake and metabolism of TNT inMedicago sativa andAllium schoenoprasum grown in hydroponic cultures containing TNT levels of 0.1 to 10 mg/1. Detectable concentrations of nitrotoluenes were significantly higher inAllium schoenoprasum than inMedicago sativa. The uptake of TNT in plants was directly related to the initial TNT level. The principal nitroaromatic components in roots and shoots of both plant species were identified as 4-ADNT and 2-ADNT in equal amounts, with substantially less TNT.

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Literature

  • Amerkhanova, N. N.;R. P. Naumova: 2,4,6-Trinitrotoluene as a source of nutrition for bacteria. Microbiology47, 318 (1978)

    Google Scholar 

  • Broughton, W. J.;M. J. Dilworth: Control of leghaemoglobin in snake beans. Biochem. J. 125, 1075 (1971)

    CAS  Google Scholar 

  • Cataldo, D. A.; S. D. Harvey; R. J. Fellows; R. M. Bean; B. D. McVeety: An evaluation of the environmental fate and behavior of munitions material (TNT, RDX) in soil and plant systems. Pacific Northwest laboratories, Richland, Report AD-A223 546 (1989)

  • Djerassi, L. S.;L. Vitany: Haemolytic episode in G6-PD deficient workers exposed to TNT. Brit. J. Ind. Med. 32, 54 (1975)

    CAS  Google Scholar 

  • Folsom, B. L.; J. C. Pennington; S. L. Teeter; M. R. Barton; J. A. Bright: Effects of soil pH and treatment level on persistence and plant uptake of 2,4,6-trinitrotoluene. Waterways Experiment Station, Corps of Engineers, Vicksburg, MS, Technical Report EL-88-22(1988)

  • Grant, W. M.: Toxicology of the eye. Charles C. Thomas Publishers, Springfield Illinois 1986, p. 953

    Google Scholar 

  • Hathaway, J. A.: Trinitrotoluene — a review of reported dose-related effects providing documentation for a workplace standard. J. Occupa. Med.19, 341 (1977)

    CAS  Google Scholar 

  • Harvey, S. D.;R. J. Fellows;D. A. Cataldo;R. M. Bean: Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high-performance liquid chromatography. J. Chromato. 518, 361 (1990)

    Article  CAS  Google Scholar 

  • Koss, G.; A. Lommel; I. Ollroge; I. Tesseraux; R. Haas; A. D. Kappos: Zur Toxikologie der Nitrotoluole und weiterer Nitroaromaten aus rüstungsbedingten Altlasten. Bundesgesundhbl. 527 (1989)

  • Osmon, J. L.;R. E. Klausmeier: The microbial degradation of explosives. Dev. Ind. Microbiol.14, 247 (1972)

    Google Scholar 

  • Palazzo, A. J.;D. C. Leggett: Effect and disposition of TNT in a terrestrial plant. J. Environ. Quail.15, 49 (1986)

    Article  CAS  Google Scholar 

  • Pennington, J. C.: Plant uptake of 2,4,6-trinitrotoluene, 4-amino-2,6-dinitrotoluene, and 2-amino-4,6-dinitrotoluene using 14C-labeled and unlabeled compounds. US Army Engineer Waterways Experiment Station, Miss Vicksburg: Technical Report EL-88-20th (1988)

  • Preuss, A.;J. Fimpel;G. Diekert: Anaerobic transformation of 2,4,6-trinitrotoluene (TNT). Arch. Microbiol.159, 345 (1993)

    Article  CAS  Google Scholar 

  • SAS Institute: SAS/STAT Guide for Personal Computers, Version 6.03 Edition 1988

  • Schott, C. D; E. G. Worthley: The toxicity of TNT and related wastes to an aquatic flowering plant,Lemna perpusilla. Edgewood Arsenal, Aberdeen Prov. Ground, MD. Techn. report 74016. AD-778158 (1974)

  • Smock, L. A.;D. L. Stoneburger;J. R. Clark: The toxic effects of trinitrotoluene (TNT) and its primary degradation products on two species of algae and the fathead minnow. Water Res10, 537 (1976)

    Article  CAS  Google Scholar 

  • Spanggord, R. J.;T. Mill;T.-W. Chou;W. R. Maybe;J. H. Smith;S. Lee: Environmental fate studies on certain munition wastewater constituents. Final report. Phase II, Laboratory studies, SRI International, Menlo Park. CA (1980)

    Google Scholar 

  • Tucker, W. L.; W. L. Banwart; T. C. Granato; J. J. Hassett: Screening for plant tolerance to TNT. Report for USATHAMA by CERL and the University of Illinois (1989)

  • V. Löw, E.;L. Kaminski;W. Neumeier;R. Haas;K. Steinbach: Mikrobieller Abbau von Nitroaromaten aus einer ehemaligen Sprengstoffproduktion. Forum Städte-Hygiene40, 347 (1989)

    Google Scholar 

  • Whipps, J. M.: Carbon economy. In:J. M. Lynch, The rhizosphere. John Wiley & Sons, Chichester 1990, pp. 59

    Google Scholar 

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Görge, E., Brandt, S. & Werner, D. Uptake and metabolism of 2,4,6-trinitrotoluene in higher plants. Environ Sci & Pollut Res 1, 229–233 (1994). https://doi.org/10.1007/BF02986535

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  • DOI: https://doi.org/10.1007/BF02986535

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