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The suitability of gastropods as biomarkers

Induction of ethoxy-, pentoxy- and benzoxyresorufin-O-dealkylase in limnetic gastropods via aroclor 1254

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Abstract

The measurement and induction of mixed function oxyigenases (MFOs) of limnetic gastropods were investigated to estimate their suitability as biomarkers. A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin-O-dealkylase-activity (BROD). Optimal measuring conditions of these activities were investigated in preparations of the digestive gland of 4 species of limnetic gastropods. Results indicate that MFO-activity inhibiting substances are accumulating in the microsomal pellet, the fraction commonly used for the measurement of MFO-activities. Therefore, the fraction used for induction studies was the postmitochondrial supernatant (PMS). EROD, PROD, and BROD activity ofPlanorbis planorbis andPlanorbis carinatus were measured after a treatment with Aroclor 1254 for 1–17 days. Maximal induction of EROD and PROD were 6 and 10 times the value in the control group ofP. carinatus, respectively induction inP. planorbis was lower. BROD-activity could not been measured inP. planorbis. InP. carinatus, BROD-activity increased to a maximum of 2 times after traetment with Aroclor 1254. The level of induction of EROD-activity is comparable to results described in analog studies with fish.

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Authors and Affiliations

  1. Institute of Ecological Chemistry, GSF, D-85758, Neuherberg, Germany

    Harald Meimberg & Antonius Kettrup

  2. Institute of Freshwater and Fish Ecology, Müggelseedamm 310, D-12587, Berlin, Germany

    Christiane Schrenk & Christian Steinberg

  3. Institute of Zoology, LMU Munich, Karlstr. 23, D-80333, München, Germany

    Albert Klarenberg

Authors
  1. Harald Meimberg
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  2. Christiane Schrenk
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  3. Christian Steinberg
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  4. Albert Klarenberg
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  5. Antonius Kettrup
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Meimberg, H., Schrenk, C., Steinberg, C. et al. The suitability of gastropods as biomarkers. Environ. Sci. & Pollut. Res. 4, 183–188 (1997). https://doi.org/10.1007/BF02986340

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  • DOI: https://doi.org/10.1007/BF02986340

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