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Inactivation of the CDKN2/pl6 gene induced by methylation at 5′-CpG Island and its relation to lung cancer

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  • Published:
Chinese Journal of Cancer Research

Abstract

Objectives: To investigate the methylation status of the CDKN2/pl6 gene 5′-CpG island, and study the relationship between the CDKN2/pl6 gene inactivation by methylation and lung cancer. Methods: Genomic DNA extracted from the specimens of lung cancer and normal lung tissue was digested with methylation-sensitive endonucleases, and Southern blotting was used to analyze the methylation status of the CDKN2/pl6 gene in 89 cases of lung cancer and 10 cases of normal lung tissue. Results: In 89 cases of lung cancer studied, the CDKN2/pl6 gene was shown to be methylated in 21 cases with the total methylation rate of 23.6% (21/89), in which there were 15 cases (16.9%) methylated atSmaI sites, 12 cases (13.5%) atSmaI sites and 6 cases at bothSmaI andSacII sites. The methylation of the CDKN2/pl6 gene occurred in 17 among 42 of pl6 protein negative cases of lung cancer with a rate of 40.5% (17/42), and in 3 among 47 of pl6 protein positive cases with a rate of 6.4% (3/47). The CDKN2/pl6 gene was not methylated in 10 cases of normal lung tissue. Conclusion: The aberrant methylation of the CDKN2/pl6 gene 5′-CpG island is probably an important mechanism of the gene inactivation, it may be involved in the genesis and progress of lung cancer.

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Authors and Affiliations

  1. Department of Molecular Biology, Cancer Center of the Chinese People’s Liberation Army (PLA), Nanjing 81st Hospital, 210002, Nanjing

    Su Chang-qing

  2. Department of Cardiothoracic Surgery, Cancer Center of the Chinese People’s Liberation Army (PLA), Nanjing 81st Hospital, 210002, Nanjing

    Ye Yu-kun & Wang Dong

  3. Department of Biology, Dongnan University Medical College, 210009, Nanjing

    Shan Xiang-nian

Authors
  1. Su Chang-qing
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  2. Ye Yu-kun
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  3. Wang Dong
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  4. Shan Xiang-nian
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Correspondence to Su Chang-qing.

Additional information

Foundation item: This work was supported by a grant from the National 9th Five-Year Plan Key Project (No. 96-906-01-18) and the National Natural Science Foundation of China (No. 39670714).

Biography: SU Chang-qing (1964-), doctor of medicine, associate professor, Cancer Center of the Chinese People’s Liberation Army (PLA), Nanjing 81st Hospital, majors in molecular biology of cancer.

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Su, Cq., Ye, Yk., Wang, D. et al. Inactivation of the CDKN2/pl6 gene induced by methylation at 5′-CpG Island and its relation to lung cancer. Chin J Cancer Res 13, 157–161 (2001). https://doi.org/10.1007/BF02983875

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  • DOI: https://doi.org/10.1007/BF02983875

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