Skip to main content
SpringerLink
Log in

Host genotype- and temperature-dependent colonizationof In vitro carnation callus cultures byFusarium oxysporum f.sp.dianthi race 2

  • Research Paper
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Differentin vivo resistance/susceptibility levels of 14 carnation cultivars toFusarium oxysporum f.sp.dianthi race 2, the causal agent of Fusarium wilt disease of carnation, were also expressed in anin vitro system and assayed as the degree of fungal colonization of callus cultures at 20° C. Temperature influenced thein vitro expression of carnation resistance. An incubation temperature of 27° C increased the colonization of calli derived from both the susceptible (‘Corrida’ and ‘Ambra’) and the resistant (‘Pulcino’ and ‘Pallas’) cultivars. At 15°C, the colonization of calli derived from Pulcino and Pallas diminished significantly more than for Ambra and Corrida. Inhibition of fungal growth on resistant calli was correlated to retardation in hyphal development. Both scanning electron microscopy and light microscopy observations showed that hyphae did not penetrate into carnation cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Agresti, A. (1990) Categorical Data Analysis. Wiley, New York, NY.

    Google Scholar 

  2. Arai, M. and Takeuchi, M. (1993) Influence of Fusarium wilt toxin(s) on carnation cells.PlantCell Tissue Organ Cult. 34:287–293.

    Article  CAS  Google Scholar 

  3. Baayen, R.P., Schoffelmeer, E.A.M., Toet, S. and Elgersma, D.M. (1997) Fungalpolygalacturonase activity reflects susceptibility of carnation cultivars to Fusarium wilt.Eur.J. Plant Pathol. 103:15–23.

    Article  CAS  Google Scholar 

  4. Baayen, R.P. and Schrama, R.M. (1990) Comparison of five stem inoculation methods withrespect to phytoalexin accumulation and Fusarium wilt development in carnation.Neth. J. PlantPathol. 96:315–320.

    Article  Google Scholar 

  5. Baayen, R.P., Sparnaaij, L.D., Jansen, J. and Niemann, G.J. (1991) Inheritance of resistancein carnation againstFusarium oxysporum f.sp.dianthi races 1 and 2, in relation to resistancecomponents.Neth. J. Plant Pathol. 97:73–86.

    Article  Google Scholar 

  6. Baker, R.R., Nelson, P.E. and Lawson, R.H. (1985) Carnation,in: Strider, D.L. [Ed.] Diseasesof Floral Crops. Vol. 1, pp. 507–563. Praeger Publishers, Westport, CT, USA.

    Google Scholar 

  7. Balass, M., Cohen, Y. and Bar-Joseph, M. (1993) Temperature-dependent resistance to downymildew in muskmelon: structural responses.Physiol. Mol. Plant Pathol. 43:11–20.

    Article  Google Scholar 

  8. Ben-Yephet, Y, Reuven, M., Lampel, M., Szmulewich, Y., Nitzani, Y. and Mor, Y. (1992)Susceptibility reaction of cultivars toFusarium oxysporum f.sp.dianthi in an infested field andin spore suspension.ActA Hortic. 307:83–88.

    Google Scholar 

  9. Ben-Yephet, Y., Reuven, M., Zviebil, A. and Shtienberg, D. (1996) Effects of initial inoculumand cultivar resistance on incidence of Fusarium wilt and population densities ofFusariumoxysporum f.sp.dianthi on carnation and in soil.Phytopathology 86:751–756.

    Article  Google Scholar 

  10. Ben-Yephet, Y, Reuven, M., Zviebil, A. and Shtienberg, D. (1996) Effects of abiotic variables onthe response of carnation cultivars toFusarium oxysporum f.sp.dianthi. Plant Pathol. 45:98–105.

    Article  Google Scholar 

  11. Ben-Yephet, Y. and Shtienberg, D. (1994) Effect of solar radiation and temperature on Fusariumwilt in carnation.Phytopathology 84:1416–1421.

    Article  Google Scholar 

  12. Ben-Yephet, Y. and Shtienberg, D. (1997) Effects of the host, the pathogen, the environment andtheir interactions on Fusarium wilt carnation.Phytoparasitica 25:207–216.

    Google Scholar 

  13. Buiatti, M., Marcheschi, G., Ventura, R., Bettini, P., Bogani, P., Morpurgo, R., Nacmias, B. andPellegrini, M.G. (1987)In vitro response to Fusarium elicitor and toxic substances in crossesbetween resistant and susceptible carnation cultivars.Plant Breeding 98:346–348.

    Article  Google Scholar 

  14. Buiatti, M., Scala, A., Bettini, P., Nascari, G., Morpurgo, R., Bogani, P., Pellegrini, G., Gimelli, F. and Ventura, R. (1985) Correlation betweenin vivo resistance toFusarium andin vitroresponse to fungal elicitors and toxic substances in carnation.Theor. Appl. Genet. 70:42–47.

    Article  CAS  Google Scholar 

  15. Demmink, J.F., Baayen, R.P. and Sparnaaij, L.D. (1989) Evaluation of the virulence of races 1,2 and 4 ofFusarium oxysporum f.sp.dianthi in carnation.Euphytica 42:55–63.

    Article  Google Scholar 

  16. Gabbrielli, R., Gori, P. and Scala, A. (1995) Ni toxicity on carnation(Dianthus caryophyllusL. cv. Corrida) cell cultures: selection of Ni tolerant lines and effects of Ca and Mg.Plant Sci.104:225–230.

    Article  CAS  Google Scholar 

  17. Garibaldi, A. (1975) Race differentiation inFusarium oxysporum f.sp.dianthi (Prill, and Delacr.)Snyd. and Hans. First contribution.Meded. Rijksfac. Landbouwwet. Gent. 40:531–537.

    Google Scholar 

  18. Garibaldi, A. (1977) Race differentiation inFusarium oxysporum f.sp.dianthi and varietalsusceptibility.Acta Hortic. 71:97–101.

    Google Scholar 

  19. Garibaldi, A. (1978) Susceptibility of carnation to Italian isolates ofFusarium oxysporum f.sp.dianthi andFusarium oxysporum f.sp.redolens. Proc. Eucarpia Meeting on Carnation andGerbera (Alassio, Italy), pp. 141–148.

    Google Scholar 

  20. Garibaldi, A. (1983) Resistenza di cultivar di garofano nei confronti di otto patotipi diFusariumoxysporum f.sp.dianthi (Prill. and Delacr.) Snyd. and Hans.Riv. Ortoflorofrutt. It. 67:261–270.

    Google Scholar 

  21. Harling, R. and Taylor, G.S. (1985) A light microscope study of resistant and susceptiblecarnations infected withFusarium oxysporum f.sp.dianthi. Can. J. Bot. 63:638–646.

    Article  Google Scholar 

  22. Harling, R., Taylor, G.S., Matthews, P. and Arthur, A.E. (1988) The effect of temperature onsymptom expression and colonization in resistant and susceptible carnation cultivars infectedwithFusarium oxysporum f.sp.dianthi. J. Phytopathol. 121:103–117.

    Article  Google Scholar 

  23. Latunde-Dada, A.O., Dixon, R.A. and Lucas, J.A. (1987) Induction of phytoalexin biosyntheticenzymes in resistant and susceptible lucerne callus lines infected withVerticillium albo-atrum.Physiol. Mol. Plant Pathol. 31:15–23.

    Article  CAS  Google Scholar 

  24. Latunde-Dada, A.O. and Lucas, J.A. (1986) Influence of temperature on host resistance andfungal sensitivity to medicarpin in lucerne callus lines infected withVerticillium albo-atrum.Physiol. Mol. Plant Pathol. 28:89–97.

    CAS  Google Scholar 

  25. Matthews, P. (1978) Variation in English isolates ofFusarium oxysporum f.sp.dianthi. Proc.Eucarpia Meeting on Carnation and Gerbera (Alassio, Italy), pp. 115–126.

    Google Scholar 

  26. Miller, S.A. (1985) Tissue culture methods in phytopathology: Fungi.in: Dixon, R.A. [Ed.]Plant Cell Culture, A Practical Approach. pp. 215–229. IRL Press, Oxford, UK.

    Google Scholar 

  27. Niemann, G.J. (1992) The mechanism of resistance of carnation to wilt diseases.Acta Hortic.307:29–36.

    Google Scholar 

  28. Niemann, G.J., Baayen, R.P. and Boon, J.J. (1990) Localization of phytoalexin accumulationand determination of changes in lignin and carbohydrate composition in carnation(Dianthuscaryophyllus L.) xylem as a consequence of infection withFusarium oxysporum f.sp.dianthi,by pyrolysis-mass spectrometry.Neth. J. Plant Pathol. 96:133–153.

    Article  CAS  Google Scholar 

  29. SAS Institute Inc. (1989) SAS/STAT User’s Guide, Version 6,4th ed. Vol. 2. Cary, NC, USA.

    Google Scholar 

  30. Scala, A., Comparini, C, Tegli, S., Ragazzi, A., Dellavalle, I., Mittempergher, L. and Lagazio, C. (1996)In vitro analysis of the interaction between elm andOphiostoma ulmi orO. novo-ulmi.Petria 6:91–103.

    Google Scholar 

  31. Sparnaaij, L.D. and Demmink, J.F. (1977) Progress towardsFusarium resistance in carnations.Acta Hortic. 71:107–113.

    Google Scholar 

  32. Tegli, S., Biancalani, I., Bonati, C. and Scala, A. (1987) The differential elicitation ofthe hypersensitive response in carnation resistant and susceptible cultivars tissue culturesby elicitor fromFusarium oxysporum f.sp.dianthi race 2.Proc. 7th Congr. MediterraneanPhytopathological Union (Granada, Spain), pp. 29–30.

    Google Scholar 

  33. Tramier, R., Antonini, A., Bettachini, A. and Metay, C. (1983) Studies on Fusarium wiltresistance in carnation.Acta Hortic. 141:49–54.

    Google Scholar 

  34. Van der Bruggen, P., Grégoire, D. and Maraite, H. (1990) Temperature-induced alterations inthe expression of susceptibility of Cassava toColletotrichum gloeosporioides f.sp.manihotis. J.Phytopathol. 130:46–58.

    Article  Google Scholar 

  35. Van der Plank, J.E. (1986) Specific susceptibility and specific feeding in gene-per-gene systems.Adv. Plant Pathol. 5:199–223.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Patologia e Zoologia Forestale e Agraria, Università di Firenze, 1-50144, Firenze, Italy

    A. Scala, S. Tegli & V. Goggioli

  2. Dipartimento di Statistica “Giuseppe Parenti”, Università di Firenze, 1-50134, Firenze, Italy

    C. Lagazio

Authors
  1. A. Scala
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Tegli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Goggioli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Lagazio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Scala.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scala, A., Tegli, S., Goggioli, V. et al. Host genotype- and temperature-dependent colonizationof In vitro carnation callus cultures byFusarium oxysporum f.sp.dianthi race 2. Phytoparasitica 26, 213–222 (1998). https://doi.org/10.1007/BF02981436

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981436

Key Words

Search

Navigation