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Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey

  • Phytopathology/Mycology
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Abstract

During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases. Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY phytoplasma infecting Japanese plum was previously reported.

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Authors and Affiliations

  1. Dept. of Plant Protection, Agriculture Faculty, Mustafa Kemal University, 31034, Antakya, Turkey

    Gülşen Sertkaya

  2. Dept. of Biologia Applicata alla Difesa delle Piante, University of Udine, 33100, Udine, Italy

    Marta Martini, Paolo Ermacora, Rita Musetti & Ruggero Osler

Authors
  1. Gülşen Sertkaya
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  2. Marta Martini
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  3. Paolo Ermacora
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  4. Rita Musetti
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  5. Ruggero Osler
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Correspondence to Gülşen Sertkaya.

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http://www.phytoparasitica.org posting July 21, 2005.

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Sertkaya, G., Martini, M., Ermacora, P. et al. Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey. Phytoparasitica 33, 380–390 (2005). https://doi.org/10.1007/BF02981306

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  • DOI: https://doi.org/10.1007/BF02981306

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