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Occurrence and identification of grapevine phytoplasmas in main viticultural regions of Turkey

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Abstract

Intensive surveys were conducted in the main viticultural areas of Turkey in 2009-2010. Two hundred eighty nine symptomatic and 20 non-symptomatic plant samples were collected and subjected to nucleic acid extraction followed by PCR, nested PCR and RFLP analyses to detect phytoplasma presence and for their identification. The incidence rate of phytoplasma infection was 18.33% and the majority of the symptomatic grapevines were infected with grapevine yellows phytoplasmas belonging to 16SrXII-A subgroup (“bois noir”). Phytoplasmas of 16SrV group, aster yellows (16SrI-B) and pigeon pea witches’ broom (16SrIX) groups were also detected in the surveyed vineyards. Phytoplasma-associated infections were present more on wine grapevine cultivars (73.6%), such as Alphonse Lavallée, Alicante Bouschet, Chardonnay, Shiraz, Cabernet Sauvignon, Merlot, Sauvignon Blanc and Pinot Noir, compared to table grapes (26.4%), such as Bogazkere, Sirfani, Tahannebi and Emir.

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References

  • Alma, A., Soldi, G., Tedeschi, R., & Marzachì, C. (2002). Role of Hyalesthes obsoletus Signoret in transmission of grapevine Bois Noir in Italy. Petria, 12, 411–412.

    Google Scholar 

  • Angelini, E., Clair, D., Borgo, M., Bertaccini, A., & Boudon-Padieu, E. (2001). Flavescence dorée in France and Italy-occurence of closely related phytoplasma isolates and their near relationships to Palatinate grapevine yellows and alder phytoplasma. Vitis, 40, 79–86.

    CAS  Google Scholar 

  • Angelini, E. (2010). Field assestment and diagnostic methods for detection of grapevine phytoplasmas. In S. Delrot, H. O. E. Medrano, L. Bavaresco, & S. Grando (Eds.), Methodologies and Results in Grapevine Research (pp. 247–255). New York: Springer.

    Chapter  Google Scholar 

  • Avramov, Z., Gillet, J., & Laginova, M. (2008). First detection of stolbur phytoplasma grapevine (Vitis vinifera cv Merlot) affected in Bulgaria. Journal of Phytopathology, 56(2), 112–114.

    Article  Google Scholar 

  • Bayram, S., Zeybekoglu, U., Soylemezoglu, G., Canik, D., Karavin, M., Cakır, A., & Ertunc, F. (2014). Putative vectors of grapevine yellows phytoplasmas in Turkey. Phytopathogenic Mollicutes, 4(1), 22–26.

    Article  Google Scholar 

  • Canik, D., Ertunc, F., Paltrinieri, S., Condalto, N., & Bertaccini, A. (2011). Identification of different phytoplasmas infecting grapevine in Turkey. Bulletin of Insectology, 64, 226–227.

    Google Scholar 

  • Carstens, R., Petersen, Y., Stephan, D., & Burger, J. T. (2012). Incidence of aster yellows disesase in South African vineyards. (pp. 242–243). UC-Davis, CA, USA: Proceedings of the 17th Congress of ICVG.

    Google Scholar 

  • Constable, F. E. (2010). Phytoplasma Epidemiology: Grapevine as a model. In P. G. Weintraub & P. Jones (Eds.), Phytoplasmas Genomes, Plant Hosts And Vectors (pp. 188–213). London, UK: CABI.

    Google Scholar 

  • Cvrković, T., Jović, J., Mitrović, M., Krstic, O., & Toševski, I. (2014). Experimental and molecular evidence of Reptalus panzeri as a natural vector of bois noir. Plant Pathology, 63(1), 42–53.

    Article  Google Scholar 

  • Celik, H., Kunter, B., Soylemezoglu, G., Ergul, A., Celik, H., Karatas, H., Ozdemir, G., & Atak, A. (2010). Bağcılığın Geliştirilmesi Yöntem Ve Üretim Hedefleri, Ziraat Mühendisliği 7. Teknik Kongresi, 23 p, Ankara.

  • Deng, S., & Huruki, C. (1991). Amplifications of 16S rRNA genes from culturable and non-culturable mollicutes. Microbiological Methods, 14, 53–61.

    Article  CAS  Google Scholar 

  • Duduk, B., Botti, S., Ivanović, M., Krstić, B., Dukić, N., & Bertaccini, A. (2004). Identification of phytoplasmas associated with grapevine yellows in Serbia. Journal of Phytopathology, 152, 575–579.

    Article  CAS  Google Scholar 

  • Duduk, B., Tian, J. B., Contaldo, N., Fan, X. P., Paltrinieri, S., Chen, Q. F., Zhao, Q. F., & Bertaccini, A. (2010). Occurence of phytoplasmas related to stolbur and to ‘Candidatus Phytoplasma japonicum’ in woody host plants in China. Journal of Phytopathology, 158, 100–110.

    Article  CAS  Google Scholar 

  • Gthb, T. C. (2012). Gıda, Tarım ve Hayvancılık Bakanlığı, Bitki Karantinası Yönetmeliği, 55 p.

  • Gibbs, K. S., Padovan, A. C., & Mogen, B. A. (1995). Studies on sweet potato little-leaf phytoplasmas detected in sweet potato and other species growing in Northern Australia. Phytopathology, 85, 169–174.

    Article  Google Scholar 

  • Güçlü, S., & Özbek, H. (1988). Some studies on the biology of Hyalesthes obsoletus Signoret (Homoptera: Cixiidae) in the conditions of Erzurum. Türkiye Entomology Dergisi, 12, 103–111.

    Google Scholar 

  • Gundersen, D. E., & Lee, I.-M. (1996). Ultrasensitive detection of phytoplasmas by nested PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35, 144–151.

    CAS  Google Scholar 

  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    CAS  Google Scholar 

  • Karimi, M., Contaldo, N., Mahmodi, B., Duduk, B., & Bertaccini, A. (2009). Identification of stolbur related phytoplasma in grapevine showing decline symptoms in Iran. Le Progrès agricole et viticole, HS, 208-209.

  • Kuzmanovic, S., Martini, M., Ermacora, P., Ferrini, F., Starovic, M., Tosic, M., Carraro, L., & Osler, R. (2008). Incidence and molecular characterization of Flavescence dorée in grapevine cultivars in different viticultural areas in Serbia. Vitis, 47, 105–111.

    CAS  Google Scholar 

  • Lee, I.-M., Gundersen, D. E., Hammond, R. W., & Davis, R. E. (1994). Use of mycoplasma like organism (MLO) group-specific oligonucleotide primers for nested PCR assays to detect mixed–MLO infections in a single host plants. Phytopathology, 84, 559–566.

    Article  CAS  Google Scholar 

  • Lee, I.-M., Davis, R. E., Gundersen-Rindal, D. E., Davis, R. E., & Bartoszyk, I. (1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16 Sr RNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 48, 1153–1169.

    Article  CAS  Google Scholar 

  • Lessio, F., & Alma, A. (2004). Dispersal patterns and chromatic response of Scaphoideus titanus Ball (Homoptera, Cicadellidae), vector of the phytoplasma agent of grapevine flavescence dorée. Agricultural and Forest Entomology, 6, 121–127.

    Article  Google Scholar 

  • Maixner, M. (1994). Transmission of German grapevine yellows (Vergilbungskrankheit) by the planthopper Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Vitis, 33, 103–104.

    Google Scholar 

  • Maixner, M. (2011). Recent advances in Bois Noir research. Petria, 21(2/3), 17–32.

    Google Scholar 

  • Martini, M., Murari, E., Mori, N., & Bertaccini, A. (1999). Identification and epidemic distribution of two flavescence dorée related phytoplasmas in Veneto (Italy). Plant Disease, 83, 925–930.

    Article  CAS  Google Scholar 

  • Martini, M., Botti, S., Marcone, C., Marzachi, C., Casati, P., Bianco, P. A., Benedetti, R., & Bertaccini, A. (2002). Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France. Molecular Cellular Probes, 16, 197–208.

    Article  CAS  PubMed  Google Scholar 

  • Prince, J. P., Davis, R. E., Wolf, T. K., Lee, I.-M., Mogen, B. D., Dally, E. L., Bertaccini, A., Credi, R., & Barba, M. (1993). Molecular detection of diverse mycoplasmalike organisms associated with grapevine yellows and their classification with aster yellows, x-disease and elm yellows MLOs. Phytopathology, 83, 1130–1137.

    Article  CAS  Google Scholar 

  • Quaglino, F., Zhao, Y., Casati, P., Bulgari, D., Bianco, P. A., Wei, W., & Davis, R. E. (2013). ‘Candidatus Phytoplasma solani’ a novel taxon associated with stolbur and bois-noir- related diseases of plants. International Journal of Systematic Evolutionary Microbiology, 63(8), 2879–2894.

    Article  CAS  PubMed  Google Scholar 

  • Sabaté, J., Lavina, A., Legorburu, J., Fortanete, J., Perez De Obanos, J. J., Perez Marin, J. L., Reyes, J., & Batlle, A. (2007). Incidence of “Bois Noir” phytoplasma in different wine-growing regions of Spain and its relation to Hyalesthes obsoletus. Bulletin of Insectology, 60(2), 367–368.

    Google Scholar 

  • Schneider, B., Seemüller, E., Smart, C. D., & Kirkpatrick, B. C. (1995). Phylogenetic classification of plant pathogenic mycoplasmalike organisms or phytoplasmas. In S. Razin & J. G. Tully (Eds.), Molecular and Diagnostic Procedures in Mycoplasmology (pp. 369–380). New York: Academic Press.

    Chapter  Google Scholar 

  • Schvester, D., Carle, P., & Moutous, G. (1961). Sur la transmission dela flavescence dorée des vignes par une cicadelle. Comptes Rendus des Séances de l’Académie d’Agriculture de France, 47, 1021–1024.

    Google Scholar 

  • Seemüller, E., Marcone, C., Lauer, U., Ragozzino, A., & Goschl, M. (1998). Current status of molecular classification of the phytoplasmas. Journal of Plant Pathology, 80, 3–26.

    Google Scholar 

  • Sforza, R., Clair, D., Daire, X., Larrue, J., & Boudon-Padieu, E. (1998). The role of Hyalesthes obsoletus (Hemiptera: Cixiidae) in the occurrence of bois noir of grapevines in France. Journal of Phytopathology, 146, 549–556.

    Article  Google Scholar 

  • Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tuik. (2008). Tarımsal Yapı Ve Üretim. Ankara: Başbakanlık Devlet İstatistik Enstitüsü yayınları.

    Google Scholar 

  • Weintraub, P. G., & Beandland, L. (2006). Insect vectors of phytoplasmas. Annual Review of Entomology, 51, 91–111.

    Article  CAS  PubMed  Google Scholar 

  • Weintraub, P. G., & Jones, P. (2010). Phytoplasmas, Genomes, Plant Hosts And Vectors. United Kingdom: Cab International.

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to Turkish Scientific and Technological Research Council for supporting this research by Project coded COST-109O005 and also to COST Action FA0807 for founding COST-STSM-090110. Part of the work was carried out in the frame of the COST Action FA0807 ”Integrated managemant of Phytoplasma Epidemics in Different Crop Systems” at Bologna University.

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Plant Protection, Ankara University, 06110, Ankara, Turkey

    Filiz Ertunc, Didem Canik Orel, Serife Bayram & Serife Topkaya

  2. Department of Agricultural Sciences, DipSA, Plant Pathology, Alma Mater Studiorum, University of Bologna, 40100, Bologna, Italy

    Samanta Paltrinieri & Assunta Bertaccini

  3. Faculty of Agriculture, Department of Horticulture, Ankara University, 06110, Ankara, Turkey

    Gokhan Soylemezoglu

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  1. Filiz Ertunc
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  2. Didem Canik Orel
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Correspondence to Filiz Ertunc.

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Ertunc, F., Orel, D.C., Bayram, S. et al. Occurrence and identification of grapevine phytoplasmas in main viticultural regions of Turkey. Phytoparasitica 43, 303–310 (2015). https://doi.org/10.1007/s12600-014-0449-7

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  • DOI: https://doi.org/10.1007/s12600-014-0449-7

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