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Exploration for anti-insect properties of lectin from seeds of soybean (Glycine max) usingBactrocera cucurbitae as a model

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Abstract

Lectin fromGlycine max L. was extracted and purified by affinity chromatography using asialofetuin-linked porous amino-activated silica beads. The concentration-dependent effect of lectin was studied on freshly laid eggs (0–8 h old) of the melon flyBactrocera cucurbitae (Coquillett); lectin failed to influence egg hatching. However, treating second instar larvae (64–72 h old) with increasing concentrations of lectin significantly reduced the development period, number of pupae and number of emergingB. cucurbitae, and was negatively correlated with the increase in the lectin concentration. The LC50 value, 54µg ml−1, was calculated on the basis of adult emergence. Treatment of the larvae (64–72 h old) with the LC50 concentration resulted in a decrease in pupal weight. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (glutathione S-transferase) was assayed in second instar larvae at the LC50 concentration of lectin after exposure for 24, 48 and 72 h. The activity of esterases increased significantly (P<0.01) at the three exposure intervals, whereas the activities of the three other hydrolyses and the transferases were significantly suppressed (P<0.01).

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References

  1. Bandyopadhyay, S., Roy, A. and Das, S. (2001) Binding of garlic (Allium sativum) leaf lectin to the gut receptors of homopteran pests is correlated to its insecticidal activity.Plant Sci. 161:1025–1033.

    Article  CAS  Google Scholar 

  2. Bergmeyer, H.U. (1974) Methods of Enzyme Analysis. Vol. 1. Academic Press, New York, NY.

    Google Scholar 

  3. Boulter, D., Croy, R.R.D., Ellis, R.J., Evans, I.M., Gatehouse, A.M.R., Gatehouse, J.A.et al. (1986) Isolation of genes involved in pest and disease resistance.in: Magnien, E. [Ed.] Report of the EEC Biomolecular Engineering Programme. Martinus Nijhoff/Junk, the Hague, the Netherlands. pp. 715–725.

    Google Scholar 

  4. Chien, C. and Dauterman, W.C. (1991) Studies on glutathione S-transferase inHelicoverpa (=Heliothis)zea.J. Insect Biochem. 21:857–864.

    Article  CAS  Google Scholar 

  5. Czapla, T.H. and Lang, B.A. (1990) Effect of plant lectins on the larval development of European corn borer (Lepidoptera: Pyralidae) and southern corn rootworm (Coleoptera: Chrysomelidae).J. Econ. Entomol. 83:2480–2485.

    Google Scholar 

  6. Devorshak, C. and Roe, R.M. (1999) The role of esterase in insecticide resistance.Rev. Toxicol. 2:501–537.

    Google Scholar 

  7. Eisemann, C.H., Donaldson, R.A., Pearson, R.D., Cadogan, L.C., Vuocolo, T. and Tellam, R.L. (1994) Larvicidal activity of lectins onLucilia cuprina: Mechanism of action.Entomol. Exp. Appl. 72:1–10.

    Article  CAS  Google Scholar 

  8. Enayati, A.A., Ranson, H. and Hemingway, J. (2005) Insect glutathione transferase and insecticide resistance.Insect Mol. Biol. 14:3.

    Article  PubMed  CAS  Google Scholar 

  9. Fasina, Y.O., Garlich, J.D., Classen, H.L., Ferket, P.R., Havenstein, G.B., Grimes, J.L.et al. (2004) Response of turkey poults to soybean lectin levels typically encountered in commercial diets.Poult Sci. 83:1559–1571.

    PubMed  CAS  Google Scholar 

  10. Gatehouse, A.M.R., Down, R.E., Powell, K.S., Sauvion, N., Rahbe, Y., Newell, C.A.et al. (1996) Transgenic potato plants with resistance to the peach-potato aphidMyzus persicae.Entomol. Exp. Appl. 79:295–307.

    Article  Google Scholar 

  11. Goldstein, I.J. and Poretz, R.D. (1986) Isolation, physiochemical characterization and carbohydrate-binding specificity of lectins.in: Liener, I.E., Sharon, N. and Goldstein, I.J. [Eds.] The Lectins: Properties, Functions and Applications in Biology and Medicine. Academic Press, New York, NY. pp. 33–247.

    Google Scholar 

  12. Gupta, J.N., Verma, A.N. and Kashyap, R.K. (1978) An improved method for mass rearing of melon fruit flyDacus cucurbitae (Coquillett).Indian J. Entomol. 40:470–471.

    Google Scholar 

  13. Harper, S.M., Hopkins, T.L. and Czapla, T.H. (1998) Effect of wheat germ agglutinin on formation and structure of the peritrophic membrane in European corn borer (Ostrinia nubilalis) larvae.Tissue Cell 30:166–176.

    Article  PubMed  CAS  Google Scholar 

  14. Janzen, D.H., Justice, B. and Liener, I.E. (1976) Insecticidal action of the phytohemagglutinin in black beans on a bruchid beetle.Science 192:795–796.

    Article  PubMed  CAS  Google Scholar 

  15. Katzenellenbogen, B.S. and Kafatos, F.C. (1971) General esterases of silkmoth moulting fluid: Preliminary characterization.J. Insect Physiol. 17:1139–1151

    Article  CAS  Google Scholar 

  16. Kaur, N., Singh, J. and Kamboj, S.S. (2002) Affinity purification and characterization of a seed lectin fromCrotalaria medicaginea.Indian J. Biochem. Biophys. 39:49–54.

    CAS  Google Scholar 

  17. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 277:680–685.

    Article  Google Scholar 

  18. Law, I.J. and Kfir, R. (1997) Effect of mannose-binding lectin from peanut and pea on the stem borerChilo partellus.Entomol. Exp. Appl. 82:261–265.

    Article  CAS  Google Scholar 

  19. Li, Z., Li, D. and Qiao, S. (2003) Effects of soybean agglutinin on nitrogen metabolism and on characteristics of intestinal tissues and pancreas in rats.Arch. Tierernaehr. 57:369–380.

    CAS  Google Scholar 

  20. Lis, H. and Sharon, N. (1986) Biological properties of lectins.in: Liener, I.E., Sharon, N. and Goldstein, I.J. [Eds.] The Lectins: Properties, Functions and Applications in Biology and Medicine. Academic Press Inc., New York, NY. p. 152.

    Google Scholar 

  21. Lowry, O.H., Rosebrough, N.J., Farr, A.R. and Randall, R.J. (1951) Protein measurements with folin-phenol reagent.J. Biol. Chem. 193:265–275.

    PubMed  CAS  Google Scholar 

  22. Machuka, J., Okeola, O.G., Chrispeels, M.J. and Jackai, L.E.N. (2000) The African yam bean seed lectin affects the development of the cowpea weevil but does not affect the development of larvae of the legume pod borer.Phytochemistry 53:667–674.

    Article  PubMed  CAS  Google Scholar 

  23. Machuka, J., Van Damme, E.J.M., Peumans, W.J. and Jackai, L.E.N. (1999) Effect of plant lectins on larval development of the legume pod borer,Maruca vitrata.Entomol. Exp. Appl. 93:179–187.

    Article  CAS  Google Scholar 

  24. McIntyre, R.J. (1971) A method for measuring activities of acid phosphatases separated by acrylamide gel electrophoresis.Biochem. Genet. 5:45–50.

    Article  Google Scholar 

  25. Melander, M., Åhman, I., Kamnert, I. and Strömdahl, A.C. (2003) Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae.Transgenic Res. 12:555–567.

    Article  PubMed  CAS  Google Scholar 

  26. Mukanganyama, S., Figueroa, C.C., Hasler, J.A. and Niemeyer, H.M. (2003) Effects of DIMBOA on detoxification enzymes of the aphidRhopalosiphum padi (Homoptera: Aphididae).J. Insect Physiol. 49:223–229.

    Article  PubMed  CAS  Google Scholar 

  27. Murdock, L.L. and Shade, R.E. (2002) Lectins and protease inhibitors as plant defenses against insects.J. Agric. Food Chem. 50:6605–6611.

    Article  PubMed  CAS  Google Scholar 

  28. Powell, K.S., Gatehouse, A.M.R., Hilder, V.A., Van Damme, E.J.M., Peumans, W.J., Boonjawat, J.et al. (1995) Different antimetabolic effects of related lectins towards nymphal stages ofNilaparvata lugens.Entomol. Exp. Appl. 75:61–65.

    Article  CAS  Google Scholar 

  29. Reisfeld, R.A., Lewis, O.J. and William, D.E. (1962) Disc electrophoresis of basic proteins and peptides on polyacrylamide gels.Nature 1451:281–283.

    Article  Google Scholar 

  30. Rup, P.J. and Kaur, R. (1993) Influence of some plant growth regulators on the hydrolytic enzymes of banana fly,Zaprionus paravittiger (Godbole and Vaidya) (Diptera: Drosophilidae).Pest Manag. Econ. Zool. 1:96–99.

    Google Scholar 

  31. Shangary, S., Singh J., Kamboj, S.S., Kamboj, K.K. and Sandhu, R.S. (1995) Purification and properties of four monocot lectins from the family Araceae.Phytochemistry 40:449–455.

    Article  PubMed  CAS  Google Scholar 

  32. Shukle, R.H. and Murdock, L.L. (1983) Lipoxygenase, trypsin inhibitor, and lectin from soybeans: Effects on larval growth ofManduca sexta (Lepidoptera: Sphingidae).Environ. Entomol. 12:787–791.

    CAS  Google Scholar 

  33. Srivastava, B.G. (1975) A chemically defined diet forDacus cucurbitae (Coquillett) larvae under aseptic conditions.Entomol. News Lett. 5:24.

    Google Scholar 

  34. Yu, S.J. and Abo-Elghar, G.E. (2000) Allelochemicals as inhibitors of glutathione S-transferase in the fall armyworm.Pestic. Biochem. Physiol. 68:173–183.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Dept. of Zoology, Insect Physiology Laboratory, Guru Nanak Dev University, 143005, Amritsar, India

    Kuljinder Singh & Pushpinder J. Rup

  2. Dept. of Molecular Biology and Biochemistry, Protein Laboratory, Guru Nanak Dev University, 143005, Amritsar, India

    Manpreet Kaur & Jatinder Singh

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  1. Kuljinder Singh
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  2. Manpreet Kaur
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  3. Pushpinder J. Rup
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  4. Jatinder Singh
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Correspondence to Pushpinder J. Rup.

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http://www.phytoparasitica.org posting Sept. 13, 2006.

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Singh, K., Kaur, M., Rup, P.J. et al. Exploration for anti-insect properties of lectin from seeds of soybean (Glycine max) usingBactrocera cucurbitae as a model. Phytoparasitica 34, 463–473 (2006). https://doi.org/10.1007/BF02981200

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