Abstract
Methods for measuring the activities of acid phosphatases with the same substrate, alpha-naphthyl acid phosphate, both before and after acrylamide gel electrophoresis are described. The gel assay, which involves elution of the precipitated dye complex, can be used to measure the length of linear reaction rates of the enzymes separated in gels. It is possible with the use of these methods both to determine the effect of electrophoresis on the activity of acid phosphatases and to correlate the amount of precipitated dye with the area under the peak on a densitometric tracing of the same band.
Similar content being viewed by others
References
Allen, J. M., and Gockerman, J. (1964). Electrophoretic separation of multiple forms of particle associated acid phosphatase. Ann. N. Y. Acad. Sci. 121 616.
Allen, R. C., and Moore, D. J. (1966). Sex associated quantitative differences in the plasma esterases of inbred mice. Endocrinology 78 655.
Allen, R. C., Popp, R. A., and Moore, D. J. (1966). Separation and relative quantitation of mouse plasma esterases with disc electrophoresis. J. Histochem. Cytochem. 13 249.
Burstone, M. S. (1962). Enzyme Histochemistry and Its Application in the Study of Neoplasma, Academic Press, New York.
Dietz, A. A., and Lubrano, T. (1967). Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis. Anal. Biochem. 20 246.
Doane, W. W. (1967). Quantitation of amylases in Drosophila separated by acrylamide gel electrophoresis. J. Exptl. Zool. 164 363.
Gadd, K. G. (1966). Staining of proteins on cellulose acetate. Nature 212 628.
Johns, E. W. (1967). The electrophoresis of histones in polyacrylamide gels and their quantitative determination. Biochem. J. 104 78.
Klasky, S., and Pickett, M. J. (1966). Electrophoretic determination of albumin/globulin ratios in mice. Nature 211 1298.
MacIntyre, R. J. (1966). The genetics of an acid phosphatase in Drosophila melanogaster and D. simulans. Genetics 53 461.
MacIntyre, R. J., and Dean, M. (1967). Subunits of acid phosphatase-1 in Drosophila: Reversible dissociation in vitro. Nature 214 274.
Neville, D. M. (1967). Fractionation of cell membrane protein by disc electrophoresis. Biochem. Biophys. Acta 104 78.
Raymond, S. (1964). Acrylamide gel electrophoresis. Ann. N.Y. Acad. Sci. 121 350.
Van der Decken, A. (1967). Quantitative micro-densitometric measurements of immunological precipitates in agar gel. Anal. Biochem. 18 442.
Wright, T. R. F., and Keck, K. (1961). Quantitative determination of esterase activities after starch gel electrophoresis. Anal. Biochem. 2 610.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MacIntyre, R.J. A method for measuring activities of acid phosphatases separated by acrylamide gel electrophoresis. Biochem Genet 5, 45–56 (1971). https://doi.org/10.1007/BF00485729
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00485729