Abstract
An actinomycin complex isolated from culture broth of a soil microorganism, SNUS 9305-011 has been examined by High performance liquid chromatography (HPLC). From the analysis of the fractions obtained by column chromatography of the ethyl acetate extract, three actinomycin components are confirmed. The HPLC analysis is carried out with a CN-bonded nucleosil column. Comparison of the retention times of the components with those of actinomycin D, C complex, Xoβ, and V suggests that they are different actinomycins. FAB mass spectra of the components also shows different molecular ions from those of standards and other reported actinomycins. The present work has demonstrated that actinomycin components can be separated by a CN-bonded HPLC column, and that comparison of their HPLC chromatograms with authentic samples and information on their molecular ions can be successfully employed for identification of actinomycins.
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References Cited
Mauger, A. B. and Katz, E., Antibiotics, Isolation Separation and Purification. Eds. G. H. Wagman and M. J. Weinstein, pp. 1–38, Elsevier (1978).
Mauger, A. B. and Stuart, O. A., Separation of actinomycins and biosynthetic analogues by normal and reverse-phase high performance liquid chromatography.J. Antibiot, 43, 220–221 (1990).
Kim, K.-J. and Park, K. L., Isolation and characterization of actinomycetes producing extracellular adenosine deaminase inhibitor. Yakhakhoeji, in press (1994).
Rzeszotarski, W. J. and Mauger, A. B., Reverse-phase high-pressure liquid chromatography of actinomycins.J. Chromatogr., 86, 246–249 (1973).
Smith, J. F. and Harrap, K. H., Adenosine-deaminase activity in leukemia.Brit. J. Cancer, 31, 544-(1975).
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Cho, Se., Goo, Y.M. & Kim, KJ. Identification of actinomycins by high performance liquid chromatography and fast atom bombardment mass spectrometry. Arch. Pharm. Res. 17, 424–427 (1994). https://doi.org/10.1007/BF02979119
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DOI: https://doi.org/10.1007/BF02979119