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Different distribution of the α2 Na+, K+-ATPase isoform between rat atria and ventricles

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Abstract

Rat ventricles respond with a biphasic positive inotropic effect to ouabain, low-dose and high-dose effects but rat atria with only a monophasic high dose effect. In an effect to understand the difference in response to ouabain of two tissues between rat atria and ventricles the levels of the α2-isoform of the Na+, K+-ATPase which has higher affinity for ouabain than the α2-isoform were determined by a [3H]ouabain binding assay. The yield of protein per gram wet weight was about 47 mg for atria and 100 mg for ventricles. The Kd values of ouabain for the high-affinity ouabain binding site (α2-isoform) were nearly the same (230 nM) in the atria and ventricles. However, the numbers of the α2-isoform (Bmax) per mg protein were approximately half in the atria. When the binding data were expressed in unit per gram tissue wet weight, the numbers of α2-isoform of the Na+, K+-ATPase in the rat atria could be detected by [3H]ouabain binding assay and the levels of this isoform are too low to show the low-dose effect of ouabain.

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Authors and Affiliations

  1. Department of Food and Nutrition, Shinil Christian college, 706-023, Taegu, Korea

    Jeung-Soo Lee

  2. College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea

    Shin-Woong Lee

  3. Department of Pharmacology and Cell Biophysics, University of Cincinnati, College of Medicine, 231 Bethesda Avenue, 45267-0575, Cincinnati, Ohio, U.S.A.

    Earl T. Wallick

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  1. Jeung-Soo Lee
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  2. Shin-Woong Lee
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Lee, JS., Lee, SW. & Wallick, E.T. Different distribution of the α2 Na+, K+-ATPase isoform between rat atria and ventricles. Arch. Pharm. Res. 19, 381–385 (1996). https://doi.org/10.1007/BF02976382

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  • DOI: https://doi.org/10.1007/BF02976382

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