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The detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, by gas chromatography/mass spectrometry

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Abstract

A sensitive gas chromatography/mass spectrometry method for the detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, is described. The method is based upon solvent extraction followed by derivatisation with methyl-bis-trifluoroacetamide (MBTFA), to give a trifluoroacetyl derivative. A profile of 7-aminoflunitrazepam urinary concentrations following ingestion of 0.5 to 4 mg oral doses is reported. The method has been found to be reproducible and can be used for the confirmation of flunitrazepam administration.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, Trinity College, Dulin 2

    J. Feely, P. V. Kavanagh & S. M. McNamara

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  1. J. Feely
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  2. P. V. Kavanagh
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  3. S. M. McNamara
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Feely, J., Kavanagh, P.V. & McNamara, S.M. The detection and quantitation of 7-aminoflunitrazepam, the major urinary metabolite of flunitrazepam, by gas chromatography/mass spectrometry. Ir. J. Med. Sc. 168, 189–192 (1999). https://doi.org/10.1007/BF02945851

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