Abstract
To find out the biochemical markers, crude extract of leaf protein of 11 varieties of sugacane was analysed using SDS-polyacrylamide gel electrophoresis .Variation in protein banding pattern was recorded. A total of ninteen bands of differnt molecular weight (mw) ranging from 25.5 kDa to 180 kDa were observed in differnt varieties. Band no. 2 and 14 with mw of 170 kDa and 34 kDa, respectively, were present in all high sugared and early maturing cultivars, whereas band no. 4 with molecular weight of 125kDa was observed only in the varieties of mid late maturing group. Thus the biochemical marker, i.e. specific protein band with known molecular weight may be helpful for character identification in highly polyploid and genetically complex sugarcane crop.
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References
Anonymous (1987). Annual Report. Experimental Station Hawaiian Sugar Planters’ Association, pp 28–29.
Bradford, M.M. (1976). A rapid and sensitive methode for the quantitation of microgram quantities of protien utilizing the principle of -Dye binding. Anal Biochem.,72: 248–254.
Cooke, R.J. (1980). The characterization and identification of crop cultivar by elctrophoresis. Electrophoresis,5: 59–72.
Eksomtramage, T., Paulet, F., Noyer, J.L., Fieldmann, P. and Glaszamann, J.C. (1992). Utility of isozymes in sugarcane breeding. Sugarcane,3: 14–21.
Glaszmann, J.C., Fautret, A., Niyer, J.L., Fieldmann, P. and Lanaud, C. (1989). Biochemical genetic markers in sugarcane, Theor. Appl. Genet,78: 537–543.
Hussain, A., Ramirez, H., Bushuk, W. and Roca, W. (1986). Field bean cultivars identification by electrophorogram of cotyledon protien. Euphytica,34: 729–732.
Jaccard, P. (1901). Bull. Soc. Vaudoise Sei. at.,37: 547–579.
Jones, B.L., Lookhart, G.L., Hall, S.B. and Inney, K.F. (1982). Identification of wheat cultivars by gliadin electrophoresis. Electrophorogram of 88 wheat cultivars most commonly known in US in 1979. Cereal Chemistry,59: 181–188.
Laemmli, U.K. (1970). Cleavage of structural protien during the assembly of the head of bacteriophage T4. Nature,227: 680–685.
Malhotra, S.D. (1994). Biotechnology in Sugarcane. Sugar Cane,3: 2–4.
Oza, H. (1983). The Indica Japanica different of rice cultivars, a review. Proc, 4th Int. SABRAO Cong. 117–128.
Shewry, R.R., Prath, M.M., Faulks, A.J., Parner, S. and Miflin, B.J. (1979). Identification of barley cultivars. J. Natl. Inst. Agric. Bot,15: 34–50.
Singh, R.K., Chandra, P., Singh. I.S., Singh, D.N. and Singh, S.B. (1997). Characterisation of sugarcane germplasm accesion by using protien profiles. Nat. Sym. on Biodiversity Conservation and Evolution of Plants held at Botany Dept., Allahabad Univ, India, pp. 116.
Sokal, R.R. and Sneath, P.H.A. (1963). Principle of numerical taxonomy, Freeman press, San Francisco.
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Chandra, P., Singh, R.K., Singh, I.S. et al. Possibility of identification and characterization of sugarcane cultivars on the basis of protein profiles. Sugar Tech 3, 59–62 (2001). https://doi.org/10.1007/BF02945533
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DOI: https://doi.org/10.1007/BF02945533