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Encapsulation of whole cell CGTase from concentrated broth solution

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Abstract

Most of the Cyclodextrin glucanotransferase (Gtases) which have been produced fromB. subtilis were found to be excreted from the cells during cultivation. Immobilized whole cell CGTase fromB. subtilis was prepared by encapsulating the broth solution which had been concentrated ten times with a rotary vacuum evaporator. Cyclization activity of CGTase was reduced by about 10% during the concentrating process, however, its transglycosylation activity, to convert xylitol to glucosyl-xylitol, using dextrin as glucosyl donor, increased by a factor of 3 or 5.

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Authors and Affiliations

  1. Department of Chemical Engineering, Kyungpook National University, 702-701, Taegu, Korea

    Joong-Kon Park, Joon-Hyuk Sohn & Hyung-Woo Park

  2. Department of Genetic Engineering, Kyungpook National University, 702-701, Taegu, Korea

    Yong-Hyun Lee

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  1. Joong-Kon Park
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  2. Joon-Hyuk Sohn
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  3. Hyung-Woo Park
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  4. Yong-Hyun Lee
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Correspondence to Joong-Kon Park.

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Park, JK., Sohn, JH., Park, HW. et al. Encapsulation of whole cell CGTase from concentrated broth solution. Biotechnol. Bioprocess Eng. 6, 67–71 (2001). https://doi.org/10.1007/BF02942253

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  • DOI: https://doi.org/10.1007/BF02942253

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