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Enzymatic catalysis in a supercritical fluid

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Abstract

The enzyme alkaline phosphatase, EC 3. 1. 3. 1, was found to be active in a supercritical carbo dioxide solvent system. A batch reaction of disodium p-nitrophenyl phosphate with a 0.1 vol. % water solution in supercritical CO2 at 100 atm and 35°C produced p-nitrophenol when catalyzed by alkaline phosphatase.

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Authors and Affiliations

  1. Department of Chemical Engineering College of Chemistry, University of California, 94720, Berkeley, California, USA

    T. W. Randolph, H. W. Blanch, J. M. Prausnitz & C. R. Wilke

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  1. T. W. Randolph
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  2. H. W. Blanch
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  3. J. M. Prausnitz
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  4. C. R. Wilke
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Randolph, T.W., Blanch, H.W., Prausnitz, J.M. et al. Enzymatic catalysis in a supercritical fluid. Biotechnol Lett 7, 325–328 (1985). https://doi.org/10.1007/BF01030279

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  • DOI: https://doi.org/10.1007/BF01030279

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