Abstract
The potential use of spin filter device to retainPropionibacterium acidipropionici in the bioreactor under continuous mode of fermentation and improve propionic acid productivity, was examined. The yield of propionic acid based on lactose concentration was 51% in batch and 54% in continuous (dilution rate=0.05 h−1) operation. The yield in continuous fermentation with cell retention using spin filter of 10 micron size (dilution rate=0.05 h−1) was even higher at 70% (w/w). The volumetric productivity under batch and continuous mode of operation were 0.312 g L−1 h−1 and 0.718 g L−1 h−1 respectively. Continuous fermentation with cell retention demonstrated even higher volumetric productivities at 0.98 g L−1 h−1 with out clogging problems It could be used for utilization of cheese whey to produce propionic acid at higher yield and productivities.
Similar content being viewed by others
References
Playne, M. J. (1985) Propionic and butyric acids. pp. 731, In: M. Moo-Young (ed.),Comprehensive Biotechnology, Volume 3, Pergamon, NY, USA.
Lewis, V. P. and S. T. Yang (1992a) Continuous propionic acid fermentation by immobilizedPropio ubacterium acidipropionici in a novel packed-bed bioreactor,Biotechnol. Bioeng. 40: 465–474.
Castillo, F. J. (1990) Lactose metabolism by yeasts. pp. 297–320. In: H. Verachtert and R. de Mot (eds.),Yeast Biotechnology and Biocatalysis, Marcel Dekker, NY, USA.
Siso, M. I. G. (1996) The biotechnological utilization of cheese whey: A review.Bioresource Technol. 57: 1–11.
Mawson, A. J. (1988) Yeast biomass production from acid whey permeate.Biotechnol. Lett. 10: 503–508.
Hsu, S. T. and S. T. Yang (1991) Tropionic acid fermentation of lactose byPropionibacterium acidopropionici: Effect of pH.Biotechnol. Bioeng. 38: 571–578.
Anderson, T. M., E. A. Bodie, N. Goodman and R. D. Schwartz (1986) Inhibitory effect of autoclaving whey based medium on propionic acid production byPropionibacterium shermanii.Appl. Env. Microbiol. 51: 427–428.
Lewis, V. P. and S. T. Yang (1992) Propionic acid fermentation byPropionibacterium acidipropionici. Effect of growth substrate.Appl. Microbiol. Biotechnol. 37: 437–442
Yang, S. T., H. Zhu and Y. Li (1994) Continuous propionate production from whey permeate using a novel fibrous bed reactor.Biotechnol. Bioeng. 43: 1124–1130.
Woskow, S. A. and B. A. Glatz (1991) Propionic acid production by a propionic acid-tolerant strain ofPropionibacterium acidipropionici in batch and semicontinuous fermentation.Appl. Environ. Microbiol. 57: 2821–2828.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gupta, A., Srivastava, A.K. Continuous propionic acid production from cheese whey usingin situ spin filter. Biotechnol. Bioprocess Eng. 6, 1–5 (2001). https://doi.org/10.1007/BF02942242
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02942242