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Determination of deoxynivalenol in cereals by HPLC-UV

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Abstract

A simple method for determination of deoxynivalenol (DON) in cereal samples is described. DON was extracted with methanol, the solvent evaporated, and the residue redissolved with water. This extract was purified on immunoaffinity columns. DON was determined by HPLC with UV-detection. The limits of detection (LOD) and quantification (LOQ) were 10 and 50 µg/kg, respectively.

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Czerwiecki, L., Wilczyńska, G. Determination of deoxynivalenol in cereals by HPLC-UV. Mycotox Res 19, 31–34 (2003). https://doi.org/10.1007/BF02940088

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