Abstract
A simple method for determination of deoxynivalenol (DON) in cereal samples is described. DON was extracted with methanol, the solvent evaporated, and the residue redissolved with water. This extract was purified on immunoaffinity columns. DON was determined by HPLC with UV-detection. The limits of detection (LOD) and quantification (LOQ) were 10 and 50 µg/kg, respectively.
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References
DON-Test HPLC Instruction manual (1999), Vicam, May 4: 10–14.
IARC (1993). IARC Monographs on the Evaluation of Carcinogenic Risks of Chemicals to Humans, 56: Some naturally occurring substances: some food items and constituents, heterocyclic aromatic amines and mycotoxins, Lyon, 599.
Ichinoe M, Uchiyama S et al. (1984) Trichothecene producing Fusarium in barley in Japan. In: Trichothecene and other mycotoxins. Proceedings of the International Mycotoxin Symposium. Lacey J.ed. Sydney, Australia, 21–32.
Reutter M (1999) Zearalenone and deoxynivalenol in cereals and feed stuffs of Schleswig-Holstein: Investigation of the harvest 1998. In: Proceedings of 21 st Mykotoxin Workshop, Jena, Germany, Gesellschaft für Mykotoxinforschung e.V., Bundesinstitut für gesundheitlichen Verbrauchschutz und Veterinärmedizin.
Scott P M, Trucksess M W (1997) Application of immunoaffinity columns to mycotoxin analysis. JAOAC Int. 80: 941–949.
Ueno Y (1987) Trichothecenes in food. In: Mycotoxins in food. Krogh P. ed. Academic Press: 123.
WHO Food Additives. Series 47 (2001) Safety evaluation of certain mycotoxins in food. Trichothecenes, Deoxynivalenol, T-2 and HT-2. World Health Organisation, Geneva. Appendix A: 529–558, 639–646 and Appendix B: 647–653.
WHO Food Additives. Series 47 (2001) Safety evaluation of certain mycotoxins in food. Deoxynivalenol: 420.
WHO Food Additives. Series 47 (2001) Safety evaluation of certain mycotoxins in food. Deoxynivalenol: 471–478.
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Czerwiecki, L., Wilczyńska, G. Determination of deoxynivalenol in cereals by HPLC-UV. Mycotox Res 19, 31–34 (2003). https://doi.org/10.1007/BF02940088
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DOI: https://doi.org/10.1007/BF02940088