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The pancreas of syrian golden hamsters

Age-related autoradiographic observations

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Summary

We examined the effects of aging on the uptake of tritiated thymidine (3H-TdR) by the different cell types of the pancreas of the Syrian golden hamster. Animals, 8-22-wk-old, received3H-TdR (2 μCi/gm) intraperito-neally and were sacrificed 1 h later. Pancreatic tissue from each animal was processed for autoradiography. The percentage of acinar cells labeled with3H-TdR was 1.17 ±0.26 at 8-wk-old and steadily diminished to 0.02 ±0.00 at 22 wk. The percentage of ductular and islet cells labeled with3H-TdR at 8 wk was 0.24±0.24 and 0.16±0.01, respectively and at 22 wk was 0.13 ± 0.09 and 0.18 ±0.01, respectively. In contrast with acinar cells, the percentage of ductular and islet cells labeled with3H-TdR between 10- and 20-wk-old showed considerable variability. In the major ducts in the head of the gland, the number of epithelial cells per mm duct length labeled with3H-TdR did not change throughout the study; 3.25 ±1.5 at 8 wk and 3.35 ±1.3 at 22 wk. It is concluded that acinar cell growth is an inverse function of age between 8-22 wk and that differentiated cell types in the pancreas of the Syrian golden hamster are capable of incorporating3H-TdR during the S-phase of the cell cycle.

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Authors and Affiliations

  1. Departments of Surgery, The Montreal General Hospital and McGill University, 1650 Cedar Avenue, Room 9860, Livingston Hall, H3G 1A4, Montreal, Quebec, Canada

    Lawrence Rosenberg

  2. Departments of Pathology, The Montreal General Hospital and McGill University, 1650 Cedar Avenue, Room 9860, Livingston Hall, H3G 1A4, Montreal, Quebec, Canada

    Lawrence Rosenberg & William P. Duguid

  3. Department of Surgery, The University of Michigan Medical Center, 48109, Ann Arbor, MI

    Aaron I. Vinik

Authors
  1. Lawrence Rosenberg
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  2. William P. Duguid
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  3. Aaron I. Vinik
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Rosenberg, L., Duguid, W.P. & Vinik, A.I. The pancreas of syrian golden hamsters. Int J Pancreatol 4, 273–280 (1989). https://doi.org/10.1007/BF02938463

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  • DOI: https://doi.org/10.1007/BF02938463

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