Abstract
The growth ofBacillus amyloliquefaciens in the aqueous two-phase system, made up of polyethylene glycol, dextran, and water, was investigated. Generally,Bacillus partitions in the dextran phase, but the magnitude of the separation depends largely on the overall composition of polymers in the phase system. The kinetics of growth ofBacillus amyloliquefaciens was studied in the polyethylene glycol-rich continuous phase, dextran-rich dispersed phase, and in the mixed phase. From the kinetic data it appears that increasing the overall polymer composition causes the cells to adsorp at the interface. On the other hand, partition measurements indicate that increasing polymer concentrations make the cell partitioning more one-sided. This anomaly is explained by studying the interfacial adsorption of cells via dynamic surface tension measurements.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albertsson, P-Å. (1986),Partition of Cell Particles and Macromolecules, Wiley Interscience, NY.
Kula, M-R., Kroner, K. H., and Hustedt, H. (1982),Adv. Biochem. Eng. 24, 73–118.
Carrol, B. J. (1976),Surf. Colloid Sci. 9, 1–67.
Raymond, F. D. andb Fischer, D. (1980),Biochim. Biophys. Acta. 596, 445–450.
Reitherman, R., Flanagan, S. D., and Barondes, S. H. (1973),Biochim. Biophys. Acta. 297, 193–202.
Veide, A., Lindback, T., and Enfors, S-O. (1984),Enz. Microb. Technol. 6, 325–330.
Gerson, D. F. (1980),Biochim. Biophys. Acta. 602, 269–280.
Johansson, G. and Westrin, H. (1978),Plant Sci. Lett. 13, 201–212.
Magnusson, K-E. and Johansson, G. (1977),FEMS Microbiol. Lett. 2, 225–228.
Van Alstine, J. M. (1984), PhD Dissertation, University of British Columbia.
Erickson, L. E., Humphrey, A. E., and Prokop, A. (1969),Biotechnol. Bioeng. 11, 449–466.
Erickson, L. E. and Humphrey, A. E. (1969),Biotechnol. Bioeng. 11, 467–487.
Erickson, L. E. (1969),Biotechnol. Bioeng. 11, 489–515.
Propkop, A., Erickson, L. E., and Paredes-Lopez, O. (1971),Biotechnol. Bioeng. 13, 241–256.
Kimizuka, H., Abood, L. G., Tahara, T., and Kaibara, K. (1971),J. Coll. Interf. Sci. 40(1), 27–34.
Hunsel, V. J., Bleys, G., and Joos, P. (1986),J. Coll. Interf. Sci. 114(2), 432–441.
Trujillo, E. M. (1983),Soc. Pet. Engrs. J. 23, 645–657.
Jennings, H. Y. (1975),Soc. Pet. Engrs. J. 15, 197–202.
Alam, S., Weigand, W. A., and Hong, J. (1988),Biotech. Bioengr. in press.
Alam, S. (1988), PhD Dissertation, Ilinois Institute of Technology, Chicago, IL.
Hartland, S. and Hartley, R. W. (1976),Assymetric Fluid-Liquid Interfaces, Elsevier, Amsterdam, pp. 609–611.
Fisher, D. (1981),Biochim J. 196, 1–10.
Walter, H. and Fisher, D. (1986),Partitioning in Aqueous Two-Phase Systems, Walter, H., Brooks, D. E., and Fisher, D., eds., Academic, NY, p. 378.
Magnusson, K-E and Standahl, O. (1986),Partitioning in Aqueous Two-Phase Systems, Walter, H., Brooks, D. E., and Fisher, D., eds., Academic, NY, pp. 416–446.
Sharpe, P. T. and Watts, D. J. (1984),FEBS Lett. 168, 89–92.
Sharpe, P. T. and Watts, D. J. (1984),Biosci. Rep. 4, 589–592.
Sharpe, P. T. and Watts, D. J. (1985),J. Cell Sci. 75, 339–346.
Andersson, E., Christin, J., and Hägerdal, H. (1985),Enz. Microb. Technol. 7, 333–338.
Brooks, D. E. (1973),J. Coll. Interf. Sci. 43, 714–726.
Lange, H. (1965),J. Coll. Sci. 20, 50.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alam, S., Weigand, W.A. & Hong, J. On the mechanism of growth of cells (Bacillus amyloliquefaciens) in the mixed aqueous two-phase system. Appl Biochem Biotechnol 20, 421–436 (1989). https://doi.org/10.1007/BF02936500
Issue Date:
DOI: https://doi.org/10.1007/BF02936500