Abstract
The influence of photoperiodic induction on the incorporation of uridine-3H into the shoot apices ofChenopodium rubrum was studied using the technique of autoradiography. No increase in uridine incorporation was detected either during induction lasting three days or immediately after its termination. Pyroninophylia likewise did not rise. However, changes in uridine incorporation related to morphogenetic activity during leaf formation and later during differentiation of inflorescences were well marked. The distribution of label in the nucleus immediately after three inductive cycles shows the ratio of extranucleolar to nucleolar incorporation to be higher in non-induced control plants than in induced ones.
Data from literature pointing to an activation of RNA synthesis during transition to flowering are discussed and compared with other systems where ontogenetic changes are accompanied by marked changes in RNA synthesis. It is assumed that the activation of RNA synthesis after induction is connected mainly with the activation of growth. However, inChenopodium rubrum photoperiodic induction proceeds together with limited growth and without activation of RNA synthesis.
Abstract
Metodou autoradiografie jsme studovali vliv fotoperiodické indukce na inkorporaci uridinu-3H do osních vrcholů. Nenalezli jsme průkazných rozdílů mezi indukovanými a neindukovanými rostlinami ani po třídenní fotoperiodické indukci, která plně postačuje k vyvolání květního stavu. Tím spíše jsme nemohli najít rozdíly ani v průběhu indukce. Nepozorovali jsme přitom ani zvýšení barvitelnosti pyroninem. Větší basofilie se projevila až v průběhu květní diferenciace.
Naproti tomu metoda autoradiografie velmi dobře umožnila sledování inkorporace uridinu do ruzných části meristemu v závislosti na jejich růstové aktivitě při zakládá ní listů a později při diferenciaci květenství.
Distribuce značení v jádře ukázala spíše na větší podíl jadé rkové inkorporace ve vrcholech indukovaných rostlin ve srovnání s rostlinami neindukovanými. Tento rozdíl byl z největší části dán zvětšením jadérka u indukovaných rostlin.
Jsou diskutovány nálezy aktivace syntézy RNA během přechodu ke kvetení a srovnány s jiný-mi systémy u niehž ontogenetieké změny jsou doprovázeny markantnimi změnami v syntéze RNA Je vysloven názor, že aktivace syntézy RNA souvisí především s aktivací růstu. UChenopodium rubrum fotoperiodická indukce probíhá při omezeném růstu a bez aktivace syntézy RNA.
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Seidlová, F. The incorporation of uridine-3H into the shoot apices of photoperiodically induced and non-induced plants ofChenopodium rubrum L.. Biol Plant 14, 241–248 (1972). https://doi.org/10.1007/BF02935847
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DOI: https://doi.org/10.1007/BF02935847