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Conditions for the extraction of DNA and RNA from tobacco pollen by sodium chloride and perchloric acid

Podminky extrakce DNA a RNA z tabákového pylu pomocí roztoků chloridu sodného a kyseliny chloristé

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Biologia Plantarum

Abstract

Studying the influence of the pH of 10% NaCl solutions used for the extraction of RNA and DNA on the yield of both nucleic acids, the maxima of pH were found at which both types of nucleic acids pass into extracts better than at neutral pH and do not remain in residues of the experimental material.The perchloric acid extraction temperature was also studied for obtaining the hydrolysate of nucleic acids from the trichloroacetic acid precipitate of sodium chloride extracts differing by 5 °C within the range of 35 °C to 90 °C and it was found that in this wide range almost the same amount of RNA is extracted by the method used. However, at a lower temperature, some DNA remained in the extracted residue of the trichloroacetic acid precipitate of sodium chloride extracts.

Abstract

Pŕi studiu vlivu pH roztoku 10% chloridu sodného užitého k extrakci RNK a DNK na vítěžek obou typů nukleovích kyselin byla nalezena maxima pH, pŕi nichž oba typy nukleovích kyselin lépe pŕecházejí do extraktu než pŕi neutrálním pH a nezůstávají ve zbytku pokusného materiálu po extrakci. Studovány byly také extrakční teploty pro získaní hydrolysátu nukleovích kyselin kyselinou chloristou ze sraženiny získane kyselinou trichloroctovou z extraktu v roztoku chloridu sodného, lišící se o 5 °C v rozmezí 35 °C až 90 °C. Bylo zjiatěno, že v tomto širokém rozmezí teplot se použitou metodou vyextrahuje pŕibližně stejně velkě množství RNK. Avšak pŕi nižŮích teplotách část DNK zůstává v extrahovaném zbytku sraženiny získané kyselinou trichloroctovou z extraktů v roztoku chloridu sodného.

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Abbreviations

TCA:

trichloroaoetic acid

PCA:

perchloric acid

NA:

nucleic acids

NA-P:

phosphorus of the sum of nucleic acids

RNA-P:

phosphorus of ribonucleic acid

DNA-P:

phosphorus of deoxyribonueleio acid

DPA:

diphenylamine

O:

orcinol

LPS:

Lowry positive substances

R-PEA:

residue of the TCA precipitate after PCA extraction

R-BPM:

residue of the pollen matter after NaCl extraction

OFP:

original fraction from pollen

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Authors and Affiliations

  1. Department of Genetics, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo nám. 2, Praha 6 — Dejvice, Czechoslovakia

    J. Süss

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Süss, J. Conditions for the extraction of DNA and RNA from tobacco pollen by sodium chloride and perchloric acid. Biol Plant 14, 385–389 (1972). https://doi.org/10.1007/BF02932978

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  • DOI: https://doi.org/10.1007/BF02932978

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