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Expression of the IL-2 gene in the potato (Solanum tuberosum cv. Superior)

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Abstract

We intended to examine the expression of the T-cell growth factor (human interleukin-2) so that a binary vector, pSSK-1, carrying the IL-2 gene was constructed and transferred intoA. tumefaciens for the purpose of the transformation of the potato (Solanum tuberosum cv. Superior). All of theAgrobacterium-infected potato explants were regenerated to shoots in modified MS medium and 81% of them rooted on the medium containing kanamycin (200 mg/L). Southern and Northern analysis were performed to verify the transformation events. EL-ISA test indicated that IL-2 protein was produced from IL-2-transformed potatoes. These results suggested expression and production of the IL-2 protein from the transgenic potato.

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Authors and Affiliations

  1. Department of Genetic Engineering, Chosun University, Kwang-Ju, Korea

    Yoonkyung Park & Hyeonsook Cheong

  2. Department of Biology, Chonnam National University, Kwang-Ju, Korea

    Baik Hwang

  3. Department of Food and Biotechnology, Dongshin University, Na-Ju, Korea

    Byoungsik Pyo

Authors
  1. Yoonkyung Park
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  2. Baik Hwang
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  3. Byoungsik Pyo
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  4. Hyeonsook Cheong
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Correspondence to Hyeonsook Cheong.

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Park, Y., Hwang, B., Pyo, B. et al. Expression of the IL-2 gene in the potato (Solanum tuberosum cv. Superior). Biotechnol. Bioprocess Eng. 2, 15–18 (1997). https://doi.org/10.1007/BF02932456

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