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The influence of bakers’ yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography

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Abstract

The influence of whole yeast cells (0–15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ=1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at aC/C 0=0..1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent, respectively.

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Authors and Affiliations

  1. Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Yen Mei Chow, Mohd Nordin Ibrahim & Tau Chuan Ling

  2. Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Beng Ti Tey

  3. Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Arbakariya Ariff

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  1. Yen Mei Chow
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  2. Beng Ti Tey
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  3. Mohd Nordin Ibrahim
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  4. Arbakariya Ariff
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  5. Tau Chuan Ling
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Correspondence to Tau Chuan Ling.

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Chow, Y.M., Tey, B.T., Ibrahim, M.N. et al. The influence of bakers’ yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography. Biotechnol. Bioprocess Eng. 10, 552–555 (2005). https://doi.org/10.1007/BF02932293

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  • DOI: https://doi.org/10.1007/BF02932293

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