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Molecular epidemiology of PER-1 extended spectrum β-lactamase among gram-negative bacteria isolated at a tertiary care hospital

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Abstract

Thebla PER-1 presence was sought by PCR in 289 ceftazidime resistant Gram-negative bacteria isolated atDokuz Eylul University Hospital (Turkey) between 1998 and 2003. PER-1 production rates were 32.3, 33.9, 14.9 and 37.9% in the 1998–2000 period, 2001, 2002 and 2003, respectively.bla PER-1 was detected in 46.2 and 35.9% of ceftazidime-resistantPseudomonas aeruginosa andAcinetobacter baumannii isolates, respectively. ERIC-PCR results revealed that dissemination of two endemic clones for bothP. aeruginosa (X and Y) andA. baumannii (A and B) was responsible for the high prevalence. Results of the conjugation tests and plasmid curing experiments suggested thatbla PER-1 was located on the chromosome in the representative strains. It was also shown for the first time thatbla PER-1 in a clinical isolate was associated with class-1 integron which could facilitate dissemination ofbla PER-1 among bacteria.

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Abbreviations

bla :

β-lactamase gene(s)

ERIC:

enterobacterial repetitive intergenic consensus

ESBL:

extended spectrum β-lactamase

EtmBr:

ethidium bromide

GES:

Guiana (Brazil) extended-spectrum (β-lactamase); also called IBC (integron-associated β-lactamase)

ICU:

intensive care unit

MIC:

minimum inhibitory concentration

NCCLS:

National Committee for Clinical Laboratory Standards (nowClinical and Laboratory Standards Institute, CLSI)

PCR:

polymerase chain reaction

PER-1:

secondary β-lactamase (ESBL first detected inP. aeruginosa)

SHV:

sulfanyl (‘sulfhydryl’) variable (β-lactamase)

TEM:

β-lactamase (originally found in the blood of a patient in Temoniera, Greece)

UPGMA:

unpaired group mathematical analysis

VEB:

Vietnamese extended-spectrum β-lactamase

References

  • Aktaş Z., Poirel L., Şalcioğlu M., Özcan P.E., Midilli K., Bal Ç., Anğ Ö., Nordmann P.: PER-1 and OXA-10-like β-lactamases in ceftazidime-resistantPseudomonas aeruginosa isolates from intensive care unit patients in İstanbul, Turkey.Clin.Microbiol.Infectol. 11, 193–198 (2005).

    Article  Google Scholar 

  • Amyes S.G., Gould I.M.: Trimethoprim resistance plasmids.Ann.Microbiol. 135B, 177–186 (1984).

    CAS  Google Scholar 

  • Bahar G., Erac B., Mert A., Gulay Z.: PER-1 production in a urinary isolate ofProvidencia rettgeri.J.Chemother. 16, 343–346 (2004).

    PubMed  CAS  Google Scholar 

  • Benczeová S., Adam D., Vrábelová M., Michálková-Papajová D., Kettner M.: Occurrence of endemic plasmids causing β-lactam resistance inEnterobacteriaceae in children’s university hospital in Munich.Folia Microbiol. 49, 457–464 (2004).

    Article  Google Scholar 

  • Birnboim H.C.: A rapid alkaline extraction method for the isolation of plasmid DNA.Meth.Enzymol. 100, 243–255 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Bradford P.A.: Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology and detection of this important resistance threat.Clin.Microbiol.Rev. 14, 933–951 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Claeys G., Verschraegen G., Baere T., Vaneechoutte M.: PER-1 β-lactamase-producingPseudomonas aeruginosa in an intensive care unit.J.Antimicrob.Chemother. 45, 924–925 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Danel F., Hall L.M.C., Gür D., Akalin H.E., Livermore D.M.: Transferable production of PER-1 β-lactamase inPseudomonas aeruginosa.J.Antimicrob.Chemother. 35, 281–294 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Franiczek R., Krzyzanowska B., Dolna I., Mokracka G., Szufnarowski K.: Extended-spectrum β-lactamase conferring transferable resistance to different antimicrobial agents inEnterobacteriaceae isolated from bloodstream infections.Folia Microbiol. 50, 119–124 (2005).

    Article  CAS  Google Scholar 

  • Gülay Z., Atay T., Yuluğ N.: Carbapenem hydrolysing β-lactamases in Gram-negative non-fermentative bacilli (TR).Antibiyotik ve Kemoterapi Dergisi 12, 509–513 (1998).

    Google Scholar 

  • Hoštacká A., Čižnár I., Slobodníková L., Kotulová D.: ClinicalPseudomonas aeruginosa: potential factors of pathogenicity and resistance to antimicrobials.Folia Microbiol. 51, 633–638 (2006).

    Article  Google Scholar 

  • Kado C.I., Liu S.T.: Rapid procedure for detection and isolation of large and small plasmids.J.Bacteriol. 145, 1365–1373 (1981).

    PubMed  CAS  Google Scholar 

  • Kolayli F., Gacar G., Karadenizli A., Saniç A., Vahaboğlu H.:The Study Group: PER-1 is still widespread in Turkish hospitals amongPseudomonas aeruginosa andAcinetobacter spp.FEMS Microbiol.Lett. 249, 241–245 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Levesque C., Piche L., Larose C., Roy P.H.: PCR mapping of integrons reveals several novel combinations of resistance genes.Antimicrob.Agents Chemother. 39, 185–191 (1995).

    PubMed  CAS  Google Scholar 

  • Luzzaro F., Mantengoli E., Perili M., Lombardi G., Orlandi V., Orsatti A., Amicosante G., Rossolini G.M., Toniolo A.: Dynamics of a nosocomial outbreak of multidrug-resistantPseudomonas aeruginosa producing the PER-1 extended-spectrum β-lactamase.J.Clin.Microbiol. 39, 1865–1870 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Mantengoli E., Rossolini G.M.: Tn5393d, a complex Tn5393 derivative carrying the PER-1 extended-spectrum β-lactamase gene and other resistance determinants.Antimicrob.Agents Chemother. 49, 3289–3296 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Nordmann P., Ronco E., Naas T., Duport C., Michel-Briand Y., Labia R.: Characterization of a novel extended-spectrum β-lactamase fromPseudomonas aeruginosa.Antimicrob.Agents Chemother. 37, 962–969 (1993).

    PubMed  CAS  Google Scholar 

  • Pagani L., Migliavacca R., Pallecchi L., Matti C., Giacobone E., Amicosante G., Romero E., Rossolini G.M.: Emerging extended-spectrum β-lactamases inProteus mirabilis.J.Clin.Microbiol. 40, 1549–1552 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Pagani L., Mantengoli E., Migliavacca R., Nucleo E., Pollini S., Spalla M., Daturi R., Romero E., Rossolini G.M.: Multifocal detection of multidrug-resistantPseudomonas aeruginosa producing the PER-1 extended-spectrum β-lactamase in northern Italy.J.Clin.Microbiol. 42, 2523–2529 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Pereira M., Perilli M., Mantengoli E., Luzzaro F., Toniolo A., Rossolini G.M., Amicosante G.: PER-1 extended-spectrum β-lactamase production in anAlcaligenes faecalis clinical isolate resistant to expanded-spectrum cephalosporins and monobactams from a hospital in northern Italy.Microb.Drug Resist. 6, 85–90 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Poirel L., Menuteau O., Agoli N., Cattoen C., Nordmann P.: Outbreak of extended-spectrum β-lactamase VEB-1-producing isolates ofAcinetobacter baumannii in a French hospital.J.Clin.Microbiol. 41, 3542–3547 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Poirel L., Cabanne L., Vahaboğlu H., Nordmann P.: Genetic environment and expression of the extended-spectrum β-lactamasebla PER-1 gene in Gram-negative bacteria.Antimicrob.Agents Chemother. 49, 1708–1713 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Soll D.R.: The ins and outs of DNA fingerprinting the infectious fungi.Clin.Microbiol.Rev. 13, 332–370 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Tsou M.F., Yu W.L., Hung S.W., Chi C.J., Chieh-Ju, Chen S.C., Wu L.T.: Detection of cefotaxime-resistant CTX-M-3 in clinical isolates ofSerratia marcescens.Folia Microbiol. 49, 452–456 (2004).

    Article  CAS  Google Scholar 

  • Vahaboğlu H., Öztürk R., Aygün G., Coskunkan F., Yaman A., Kaygusuz A., Leblebicioglu H., Balik I., Aydin K., Otkun M.: Widespread detection of PER-1-type extended-spectrum β-lactamases among nosocomialAcinetobacter andPseudomonas aeruginosa isolates in Turkey: a nationwide multicenter study.Antimicrob.Agents Chemother. 41, 2265–2269 (1997).

    PubMed  Google Scholar 

  • Vahaboğlu H., Coşkunkan F., Tansel O., Ozturk R., Sahin N., Koksal I., Kocazeybek B., Tatman-Otkun M., Leblebicioglu H., Ozinel M.A., Akalin H., Kocagoz S., Korten V.: Clinical importance of extended-spectrum β-lactamase (PER-1-type)-producingAcinetobacter spp. andPseudomonas aeruginosa strains.J.Med.Microbiol. 50, 642–645 (2001).

    PubMed  Google Scholar 

  • Weldhagen G.F.: Integrons and β-lactamases — a novel perspective on resistance.Internat.J.Antimicrob.Agents 23, 556–562 (2004).

    Article  CAS  Google Scholar 

  • Weldhagen G.F., Poirel L., Nordmann P.: Ambler class A extended-spectrum β-lactamases inPseudomonas aeruginosa: novel developments and clinical impact.Animicrob.Agents Chemother. 47, 2385–2392 (2003).

    Article  CAS  Google Scholar 

  • Yong D., Shin J.H., Kim S., Lim Y., Yum J.H., Lee K., Chong Y., Bauernfeind A.: High prevalence of PER-1 extended-spectrum β-lactamase-producingAcinetobacter spp. in Korea.Antimicrob.Agents Chemother. 47, 1749–1751 (2003).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35100, Izmir, Turkey

    B. Eraç

  2. Department of Microbiology and Clinical Microbiology, Dokuz Eylul University Medical School, 35340, Izmir, Turkey

    Z. Gülay

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  1. B. Eraç
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  2. Z. Gülay
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Correspondence to B. Eraç.

Additional information

This research was supported byDokuz Eylul University grant no. 03.K.B.SAG.009.

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Eraç, B., Gülay, Z. Molecular epidemiology of PER-1 extended spectrum β-lactamase among gram-negative bacteria isolated at a tertiary care hospital. Folia Microbiol 52, 535–541 (2007). https://doi.org/10.1007/BF02932116

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