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Protein synthesis elongation factor Tu present in spores ofStreptomyces coelicolor can be phosphorylatedin vitro by the spore protein kinase

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Abstract

In vitro phosphorylation of EF-Tu was shown in cell-free extract from dormant spores ofStreptomyces coelicolor by a protein kinase present in spores. EF-Tu phosphorylation was observed on both intrinsicS. coelicolor factor and externally added purified EF-Tu fromS. aureofaciens, on two isoforms. Putative serine and threonine residues as potential phosphorylation targets were determined in primary sequence and demonstrated on 3D structure model of EF-Tu.

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Author notes

  1. M. Holub

    Present address: Rhode Island Hospital, Brown Medical School, 432 Pierre M. Galletti Building, 55 Claverick St., 02903, Providence, RI, USA

Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    M. Holub, S. Bezoušková, L. Kalachová & J. Weiser

Authors
  1. M. Holub
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  2. S. Bezoušková
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  3. L. Kalachová
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  4. J. Weiser
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Correspondence to J. Weiser.

Additional information

This work was supported by theGrant Agency of the Czech Republic (grant no. 204/03/1014),Grant Agency of the Academy of Sciences of the Czech Republic (grant no. IAA 600 200 702) and byInstitutional Research Concept no. AV OZ 5020 0510.

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Holub, M., Bezoušková, S., Kalachová, L. et al. Protein synthesis elongation factor Tu present in spores ofStreptomyces coelicolor can be phosphorylatedin vitro by the spore protein kinase. Folia Microbiol 52, 471–478 (2007). https://doi.org/10.1007/BF02932106

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  • DOI: https://doi.org/10.1007/BF02932106

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