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Hybridization analysis and mapping of the celesticetin gene cluster revealed genes shared with lincomycin biosynthesis

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Abstract

The first insight into celesticetin biosynthetic gene cluster ofS. caelestis is presented. The genomic DNA of producing strain was digested, digoxigenin-labeled and hybridized with a set of probes designed according toS. lincolnensis gene sequences. Genes with high homology to the lincomycin biosynthetic genes coding for the predicted common parts of the pathway were identified inS. caelestis. Then, genomic DNA ofS. caelestis treated by a multiple digestion was hybridized with five digoxigenin-labeled probes to construct a rough restriction map. Two consecutive islands formed by the genes with a putative function in biosynthesis of the shared saccharide moiety revealed an organization similar to the lincomycin biosynthetic gene cluster. The celesticetin cluster was mapped and essential information was obtained for subsequent steps,i.e. isolation and sequence analysis of the cluster.

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Abbreviations

CBC:

celesticetin biosynthetic cluster

ccb :

celesticetin biosynthesis gene

Cel:

celesticetin

Dig:

digoxigenin

chDNA:

chromosomal DNA

LBC:

lincomycin biosynthetic cluster

Lin:

lincomycin

lmb :

lincomycin biosynthesis gene(s)

lmr :

lincomycin resistance gene(s)

Mtl:

methylthiolincosamide

ORF:

open reading frame

PCR(s):

polymerase chain reaction(s)

Ppl:

4-propyl-l-proline

S.cae. :

Streptomyces caelestis

S.coe. :

Streptomyces coelicolor

S.lin. :

Streptomyces lincolnensis

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Authors and Affiliations

  1. Institute of Microbiology, 142 20, Prague, Czechia

    L. Čermák, J. Novotná, M. Ságová-Marečková, J. Kopecký, L. Najmanová & J. Janata

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  1. L. Čermák
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  2. J. Novotná
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  3. M. Ságová-Marečková
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  4. J. Kopecký
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  5. L. Najmanová
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  6. J. Janata
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Corresponding author

Correspondence to L. Čermák.

Additional information

This work was supported by theCzech Science Foundation, grants GA204/04/0801 and GA204/05/0616, and byInstitutional Research Concept of the Institute of Microbiology, Academy of Sciences of the Czech Republic, no. AV 0Z 5020 0510.

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Čermák, L., Novotná, J., Ságová-Marečková, M. et al. Hybridization analysis and mapping of the celesticetin gene cluster revealed genes shared with lincomycin biosynthesis. Folia Microbiol 52, 457–462 (2007). https://doi.org/10.1007/BF02932104

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  • DOI: https://doi.org/10.1007/BF02932104

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