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Catabolic degradation of 4-chlorobiphenyl byPseudomonas sp. DJ-12 via consecutive reaction ofmeta-cleavage and hydrolytic dechlorination

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Abstract

Pseudomonas sp. strain DJ-12 is a bacterial isolate capable of degrading 4-chlorobiphenyl (4CBP) as a carbon and energy source. The catabolic degradation of 4CBP by the strain DJ-12 was studied along with the genetic organization of the genes responsible for the crucial steps of the catabolic degradation. The catabolic pathway was characterized as being conducted by consecutive reactions of themeta-cleavage of 4CBP, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, andmeta-cleavage of protocatechuate. ThepcbC gene responsible for themeta-cleavage of 4CBP only showed a 30 to 40% homology in its deduced amino acid sequence compared to those of the corresponding genes from other strains. The amino acid sequence of 4CBA-CoA dechlorinase showed an 86% homology with that ofPseudomonas sp. CBS3, yet only a 50% homology with that ofArthrobacter spp. However, thefcb genes for the hydrolytic dechlorination of 4CBA inPseudomonas sp. DJ-12 showed an uniquely different organization from those of CBS3 and other reported strains. Accordingly, these results indicate that strain DJ-12 can degrade 4CBP completely viameta-cleavage and hydrolytic dechlorination using enzymes that are uniquely different in their amino acid sequences from those of other bacterial strains with the same degradation activities.

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Authors and Affiliations

  1. Laboratory of Environmental and Molecular Microbiology, Department of Microbiology and Biotechnology, Chungbuk National University, 361-763, Cheongju, Korea

    Jong-Chan Chae, Sang-Ho Park & Chi-Kyung Kim

  2. Research Institute for Genetic Engineering, Chungbuk National University, 361-763, Cheongju, Korea

    Jong-Chan Chae, Sang-Ho Park & Chi-Kyung Kim

  3. Department of Fish Pathology, Yosu National University, 550-749, Yosu, Korea

    Eunheui Kim

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Correspondence to Chi-Kyung Kim.

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Chae, JC., Kim, E., Park, SH. et al. Catabolic degradation of 4-chlorobiphenyl byPseudomonas sp. DJ-12 via consecutive reaction ofmeta-cleavage and hydrolytic dechlorination. Biotechnol. Bioprocess Eng. 5, 449–455 (2000). https://doi.org/10.1007/BF02931946

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