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High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori

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Abstract

Biologically active porcine Interleukin-2(poIL-2) was produced fromin vitro andin vivo baculovirus expression systems, namely theAutographa californica nuclear polyhedrosis virus (AcNPV)-cell culture system and the Hybrid nuclear polyhedrosis virus (HyNPV)— silkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.

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Authors and Affiliations

  1. Department of Biological Product, National Institute of Animal Health, 305-0856, Tsukuba, Ibaraki, Japan

    Shigeki Inumaru & Takehiro Kokuho

  2. Nikko Branch, National Research Institute of Aquaculture, 321-1661, Nikko, Tochigi, Japan

    Takashi Yada

  3. Department of Sericulture, National Institute of Sericultural and Entomological Science, 305-8634, Tsukuba, Ibaraki, Japan

    Makoto Kiuchi

  4. Department of Insect Technology, National Institute of Sericultural and Entomological Science, 305-8634, Tsukuba, Ibaraki, Japan

    Mitsuhiro Miyazawa

Authors
  1. Shigeki Inumaru
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  2. Takehiro Kokuho
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  3. Takashi Yada
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  4. Makoto Kiuchi
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  5. Mitsuhiro Miyazawa
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Correspondence to Shigeki Inumaru.

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Inumaru, S., Kokuho, T., Yada, T. et al. High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori . Biotechnol. Bioprocess Eng. 5, 146–149 (2000). https://doi.org/10.1007/BF02931887

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  • DOI: https://doi.org/10.1007/BF02931887

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