Abstract
Growth of 2 strains ofBifidobacterium breve and 4 strains ofBifidobacterium animalis was evaluated with glucose, raffinose or a mixture of glucose and raffinose as substrates. All strains ofB. animalis and one strain ofB. breve grew more slowly on glucose than on raffinose or the mixture of glucose and raffinose; one strain ofB. breve grew more rapidly on the mixture of glucose and raffinose than on raffinose while 2 strains ofB. animalis grew faster on raffinose than on the mixture of raffinose and glucose. Both strains ofB. breve utilized glucose and raffinose simultaneously. In contrast, all strains ofB. animalis strains displayed atypical growth with rapid utilization of raffinose followed by slow utilization of glucose. The cell morphology of all strains ofB. animalis was affected by the sugar used for cultivation: tiny and rather spherical cells were observed on glucose while on media with raffinose the cells were large and had the species-specific shape. Description of preferential utilization of various saccharides by bifidobacteria can contribute to the development of new synbiotic preparations and new cultivation media for bifidobacteria.
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This study was supported by grant MSM 6046070901 of theGrant Agency of Ministry of Education, Youth, and Sports of the Czech Republic and grant 523/03/H076 of theGrant Agency of the Czech Republic.
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Trojanová, I., Vlková, E., Rada, V. et al. Different utilization of glucose and raffinose inBifidobacterium breve andBifidobacterium animalis . Folia Microbiol 51, 320–324 (2006). https://doi.org/10.1007/BF02931824
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DOI: https://doi.org/10.1007/BF02931824